論文 - 詳細
| RRC ID | 15915 |
|---|---|
| 著者 | Hirayama H, Suzuki Y, Abe M, Miyazaki M, Makita H, Inagaki F, Uematsu K, Takai K. |
| タイトル | Methylothermus subterraneus sp. nov., a moderately thermophilic methanotroph isolated from a terrestrial subsurface hot aquifer. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel methane-oxidizing bacterium, strain HTM55(T), was isolated from subsurface hot aquifer water from a Japanese gold mine. Strain HTM55(T) was a Gram-negative, aerobic, motile, coccoid bacterium with a single polar flagellum and the distinctive intracytoplasmic membrane arrangement of a type I methanotroph. Strain HTM55(T) was a moderately thermophilic, obligate methanotroph that grew on methane and methanol at 37-65 °C (optimum 55-60 °C). The isolate grew at pH 5.2-7.5 (optimum 5.8-6.3) and with 0-1 % NaCl (optimum 0-0.3 %). The ribulose monophosphate pathway was operative for carbon assimilation. The DNA G+C content was 54.4 mol% and the major fatty acids were C(16 : 0) (52.0 %) and C(18 : 1)ω7c (34.8 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain HTM55(T) was closely related to Methylothermus thermalis MYHT(T) (99.2 % 16S rRNA gene sequence similarity), which is within the class Gammaproteobacteria. However, DNA-DNA relatedness between strain HTM55(T) and Methylothermus thermalis MYHT(T) was ≤ 39 %. On the basis of distinct phylogenetic, chemotaxonomic and physiological characteristics, strain HTM55(T) represents a novel species of the genus Methylothermus, for which the name Methylothermus subterraneus sp. nov. is proposed. The type strain is HTM55(T) ( = JCM 13664(T) = DSM 19750(T)). |
| 巻・号 | 61(Pt 11) |
| ページ | 2646-2653 |
| 公開日 | 2011-11-1 |
| DOI | 10.1099/ijs.0.028092-0 |
| PMID | 21148670 |
| MeSH | Base Composition DNA, Bacterial / genetics Fatty Acids / metabolism Hot Springs / microbiology* Hot Temperature Methane / metabolism* Methanol / metabolism* Methylococcaceae / classification* Methylococcaceae / genetics Methylococcaceae / isolation & purification* Methylococcaceae / metabolism Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics |
| IF | 2.415 |
| 引用数 | 38 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 12421 JCM 13664 |