| RRC ID |
15915
|
| 著者 |
Hirayama H, Suzuki Y, Abe M, Miyazaki M, Makita H, Inagaki F, Uematsu K, Takai K.
|
| タイトル |
Methylothermus subterraneus sp. nov., a moderately thermophilic methanotroph isolated from a terrestrial subsurface hot aquifer.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel methane-oxidizing bacterium, strain HTM55(T), was isolated from subsurface hot aquifer water from a Japanese gold mine. Strain HTM55(T) was a Gram-negative, aerobic, motile, coccoid bacterium with a single polar flagellum and the distinctive intracytoplasmic membrane arrangement of a type I methanotroph. Strain HTM55(T) was a moderately thermophilic, obligate methanotroph that grew on methane and methanol at 37-65 °C (optimum 55-60 °C). The isolate grew at pH 5.2-7.5 (optimum 5.8-6.3) and with 0-1 % NaCl (optimum 0-0.3 %). The ribulose monophosphate pathway was operative for carbon assimilation. The DNA G+C content was 54.4 mol% and the major fatty acids were C(16 : 0) (52.0 %) and C(18 : 1)ω7c (34.8 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain HTM55(T) was closely related to Methylothermus thermalis MYHT(T) (99.2 % 16S rRNA gene sequence similarity), which is within the class Gammaproteobacteria. However, DNA-DNA relatedness between strain HTM55(T) and Methylothermus thermalis MYHT(T) was ≤ 39 %. On the basis of distinct phylogenetic, chemotaxonomic and physiological characteristics, strain HTM55(T) represents a novel species of the genus Methylothermus, for which the name Methylothermus subterraneus sp. nov. is proposed. The type strain is HTM55(T) ( = JCM 13664(T) = DSM 19750(T)).
|
| 巻・号 |
61(Pt 11)
|
| ページ |
2646-2653
|
| 公開日 |
2011-11-1
|
| DOI |
10.1099/ijs.0.028092-0
|
| PMID |
21148670
|
| MeSH |
Base Composition
DNA, Bacterial / genetics
Fatty Acids / metabolism
Hot Springs / microbiology*
Hot Temperature
Methane / metabolism*
Methanol / metabolism*
Methylococcaceae / classification*
Methylococcaceae / genetics
Methylococcaceae / isolation & purification*
Methylococcaceae / metabolism
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
|
| IF |
2.415
|
| 引用数 |
38
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 12421
JCM 13664 |
