| RRC ID |
15950
|
| 著者 |
Asakuma S, Hatakeyama E, Urashima T, Yoshida E, Katayama T, Yamamoto K, Kumagai H, Ashida H, Hirose J, Kitaoka M.
|
| タイトル |
Physiology of consumption of human milk oligosaccharides by infant gut-associated bifidobacteria.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
The bifidogenic effect of human milk oligosaccharides (HMOs) has long been known, yet the precise mechanism underlying it remains unresolved. Recent studies show that some species/subspecies of Bifidobacterium are equipped with genetic and enzymatic sets dedicated to the utilization of HMOs, and consequently they can grow on HMOs; however, the ability to metabolize HMOs has not been directly linked to the actual metabolic behavior of the bacteria. In this report, we clarify the fate of each HMO during cultivation of infant gut-associated bifidobacteria. Bifidobacterium bifidum JCM1254, Bifidobacterium longum subsp. infantis JCM1222, Bifidobacterium longum subsp. longum JCM1217, and Bifidobacterium breve JCM1192 were selected for this purpose and were grown on HMO media containing a main neutral oligosaccharide fraction. The mono- and oligosaccharides in the spent media were labeled with 2-anthranilic acid, and their concentrations were determined at various incubation times using normal phase high performance liquid chromatography. The results reflect the metabolic abilities of the respective bifidobacteria. B. bifidum used secretory glycosidases to degrade HMOs, whereas B. longum subsp. infantis assimilated all HMOs by incorporating them in their intact forms. B. longum subsp. longum and B. breve consumed lacto-N-tetraose only. Interestingly, B. bifidum left degraded HMO metabolites outside of the cell even when the cells initiate vegetative growth, which indicates that the different species/subspecies can share the produced sugars. The predominance of type 1 chains in HMOs and the preferential use of type 1 HMO by infant gut-associated bifidobacteria suggest the coevolution of the bacteria with humans.
|
| 巻・号 |
286(40)
|
| ページ |
34583-92
|
| 公開日 |
2011-10-7
|
| DOI |
10.1074/jbc.M111.248138
|
| PII |
S0021-9258(20)73841-X
|
| PMID |
21832085
|
| PMC |
PMC3186357
|
| MeSH |
Adult
Bifidobacteriales Infections / microbiology
Bifidobacterium / metabolism*
Carbohydrates / chemistry
Chromatography, High Pressure Liquid / methods
Female
Gastrointestinal Tract / microbiology
Humans
Infant, Newborn
Intestines / microbiology*
Japan
Milk, Human / metabolism*
Molecular Sequence Data
Monosaccharides
Oligosaccharides / chemistry
Oligosaccharides / metabolism*
Time Factors
|
| IF |
4.238
|
| 引用数 |
196
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1192
JCM 1217
JCM 1222
JCM 1254
JCM 1255
JCM 7004 |
