| RRC ID |
15952
|
| 著者 |
Morita T, Ogura Y, Takashima M, Hirose N, Fukuoka T, Imura T, Kondo Y, Kitamoto D.
|
| タイトル |
Isolation of Pseudozyma churashimaensis sp. nov., a novel ustilaginomycetous yeast species as a producer of glycolipid biosurfactants, mannosylerythritol lipids.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
An ustilaginomycetous anamorphic yeast species isolated from the leaves of Saccharum officinarum (sugarcane) in Okinawa, Japan, was identified as a novel Pseudozyma species based on morphological and physiological aspects and molecular taxonomic analysis using the D1/D2 domains of the large subunit (26S) rRNA gene and the internal transcribed spacer 1 (ITS1)-5.8S-ITS2 regions. The name Pseudozyma churashimaensis sp. nov. was proposed for the novel species, with JCM 16988(T) as the type strain. Interestingly, P. churashimaensis was found to produce glycolipid biosurfactants, a mixture of mannosylerythritol lipids (MELs), including a novel tri-acetylated derivative (MEL-A2), from glucose. The observed critical micelle concentration (CMC) and the surface tension at CMC of MEL-A2 were 1.7 × 10⁻⁶ M and 29.2 mN/m, respectively. Moreover, on a water-penetration scan, MEL-A2 efficiently formed different lyotropic liquid crystalline phases, including the lamella phase at a wide range of concentrations, indicating its excellent surface-active and self-assembling properties. The novel strain of the genus Pseudozyma should thus facilitate the application of glycolipid biosurfactants in combination with other MEL producers.
|
| 巻・号 |
112(2)
|
| ページ |
137-44
|
| 公開日 |
2011-8-1
|
| DOI |
10.1016/j.jbiosc.2011.04.008
|
| PII |
S1389-1723(11)00153-8
|
| PMID |
21606002
|
| MeSH |
Genes, rRNA
Glycolipids / biosynthesis*
Glycolipids / chemistry
Plant Leaves / microbiology
Surface-Active Agents / chemistry
Surface-Active Agents / metabolism*
Ustilaginales / genetics
Ustilaginales / isolation & purification
Ustilaginales / metabolism*
Yeasts / genetics
Yeasts / isolation & purification
Yeasts / metabolism
|
| IF |
2.366
|
| 引用数 |
31
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 16988 |
