| RRC ID |
1595
|
| Author |
Arap MA, Lahdenranta J, Hajitou A, Marini FC 3rd, Wood CG, Wright KC, Fueyo J, Arap W, Pasqualini R.
|
| Title |
Model of unidirectional transluminal gene transfer.
|
| Journal |
Mol Ther
|
| Abstract |
Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (beta-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin.
|
| Volume |
9(2)
|
| Pages |
305-10
|
| Published |
2004-2-1
|
| DOI |
10.1016/j.ymthe.2003.11.016
|
| PII |
S1525-0016(03)00363-0
|
| PMID |
14759814
|
| MeSH |
Animals
Gene Expression
Genes, Reporter / genetics
Genetic Vectors / genetics
Glycosaminoglycans / metabolism
Humans
In Vitro Techniques
Peritoneum / metabolism
Swine
Transduction, Genetic / methods*
Transduction, Genetic / standards
Transgenes / genetics
Ureter / metabolism
|
| IF |
8.986
|
| Times Cited |
4
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| DNA material |
pSK II+[X-K]nbK20 (RDB01892) |
