RRC ID |
15960
|
Author |
McTaggart LR, Lei E, Richardson SE, Hoang L, Fothergill A, Zhang SX.
|
Title |
Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
|
Journal |
J Clin Microbiol
|
Abstract |
Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.
|
Volume |
49(8)
|
Pages |
3050-3
|
Published |
2011-8-1
|
DOI |
10.1128/JCM.00651-11
|
PII |
JCM.00651-11
|
PMID |
21653762
|
PMC |
PMC3147715
|
MeSH |
Cryptococcus gattii / chemistry*
Cryptococcus gattii / classification*
Cryptococcus neoformans / chemistry*
Cryptococcus neoformans / classification*
DNA, Fungal / chemistry
DNA, Fungal / genetics
Humans
Microbiological Techniques / methods*
Mycology / methods*
Sensitivity and Specificity
Sequence Analysis, DNA / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
|
IF |
5.897
|
Times Cited |
64
|
WOS Category
|
MICROBIOLOGY
|
Resource |
General Microbes |
JCM 2466
JCM 3685
JCM 6359
JCM 7255
JCM 8115
JCM 9195 |