| RRC ID |
15961
|
| 著者 |
Nakanaga K, Hoshino Y, Era Y, Matsumoto K, Kanazawa Y, Tomita A, Furuta M, Washizu M, Makino M, Ishii N.
|
| タイトル |
Multiple cases of cutaneous Mycobacterium massiliense infection in a "hot spa" in Japan.
|
| ジャーナル |
J Clin Microbiol
|
| Abstract |
Seven body polishers working in the same "hot spa" presented with multiple red nodules and papules on their hands and forearms. A causative agent was successfully isolated from two of the subjects and from a swab sample collected from the underside of a bed cover in the body-polishing facility. The two cutaneous isolates and the environmental isolate were rapidly growing mycobacteria that formed nonphotochromogenic smooth or smooth/rough colonies on Ogawa egg slants. They were identified as Mycobacterium massiliense by multigenotypic analysis using the 16S rRNA, hsp65, and rpoB genes and the 16S-23S rRNA internal transcribed spacer (ITS) region. However, the use of the 16S rRNA gene sequence and/or DNA-DNA hybridization (DDH Mycobacteria Kit) alone would not distinguish M. massiliense from mycobacteria in the M. chelonae-M. abscessus group. The three isolates were significantly more susceptible to clarithromycin, doxycycline, and minocycline than the M. abscessus and M. bolletii reference strains. One cutaneous isolate and the environmental isolate were in a related cluster by randomly amplified polymorphic DNA PCR (RAPD-PCR). Of the several mycobacterial species found in the day spa, only M. massiliense was isolated from biopsy specimens of the skin lesions, suggesting that this bacterium is a human skin pathogen. This is the first known report of cutaneous M. massiliense infections that could not be attributed to a prior invasive procedure. This is also the first report of M. massiliense infection in Japan.
|
| 巻・号 |
49(2)
|
| ページ |
613-7
|
| 公開日 |
2011-2-1
|
| DOI |
10.1128/JCM.00817-10
|
| PII |
JCM.00817-10
|
| PMID |
21159943
|
| PMC |
PMC3043493
|
| MeSH |
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Typing Techniques
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Environmental Microbiology
Female
Humans
Japan
Microbial Sensitivity Tests
Middle Aged
Molecular Sequence Data
Mycobacterium / classification
Mycobacterium / genetics
Mycobacterium / isolation & purification*
Mycobacterium Infections / diagnosis*
Mycobacterium Infections / microbiology*
Nucleic Acid Hybridization
RNA, Ribosomal, 16S / genetics
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
Skin Diseases, Bacterial / diagnosis*
Skin Diseases, Bacterial / microbiology*
|
| IF |
5.897
|
| 引用数 |
30
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 6388
JCM 13569
JCM 15297
JCM 15300 |
