RRC ID 1597
Author Suzuki E, Murata T, Watanabe S, Kujime Y, Hirose M, Pan J, Yamazaki T, Ugai H, Yokoyama KK.
Title A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks.
Journal Oncol. Rep.
Abstract Recombinant adenoviral vectors have been developed for use as therapeutic agents and for the introduction of exogenous genes into living cells. However, the occurrence of replication-competent adenoviruses (RCA) in adenovirus stocks produced in 293 cells remains a major problem in terms of the safe use of such vectors. To overcome the problems associated with the occurrence of RCA, we have established a simple method for the simultaneous detection of amplified E1A and E1B from RCA that might contaminate adenoviral stocks. The products amplified by polymerase chain reaction (PCR) were fractionated by regular electrophoresis on agarose gels and visualized by staining with ethidium bromide. This method is rapid and inexpensive for detection of RCA in the preparation of adenoviruses.
Volume 11(1)
Pages 173-8
Published 2004-1
PMID 14654922
MeSH Adenoviridae / classification Adenoviridae / genetics* Adenovirus E1A Proteins / genetics* Adenovirus E1B Proteins / genetics* Cell Line DNA Primers / genetics DNA, Viral / genetics Electrophoresis, Agar Gel HeLa Cells Humans Polymerase Chain Reaction / methods* Recombination, Genetic Virus Replication
IF 2.976
Times Cited 9
WOS Category ONCOLOGY
Resource
DNA material AxCANLacZ (RDB01749) AxCAEGFP (forward) (RDB03343).