| RRC ID |
15977
|
| 著者 |
Terato H, Suzuki K, Nishioka N, Okamoto A, Shimazaki-Tokuyama Y, Inoue Y, Saito T.
|
| タイトル |
Characterization and radio-resistant function of manganese superoxide dismutase of Rubrobacter radiotolerans.
|
| ジャーナル |
J Radiat Res
|
| Abstract |
Rubrobacter radiotolerans is the most radio-resistant eubacterium without spore-formation in the life cycle, and its D(37) is 16,000 Gy against gamma-rays. To understand the molecular mechanism of the high radio-resistance, we purified and characterized superoxide dismutase (SOD) of this organism as enzymatic radical scavenger, and then analyzed its genetic information. The purified SOD protein formed homo-tetramerization of 24,000 Da-monomer, while maintaining its enzymatic activity against potassium cyanide and hydrogen peroxide. We obtained a partial amino acid sequence of the protein and cloned the gene from it. Sequence analysis of the cloned gene indicated that the protein showed a similarity to other bacterial manganese SODs (Mn-SODs). Sequencing for adjacent regions of the gene showed that the gene had promoter elements with an open reading frame for putative PAS/PAC sensor protein at the 5'-adjacent region. Introduction of the gene into Escherichia coli cells lacking intrinsic SOD genes restored the cellular enzymatic activity and resistance to methyl viologen, indicating the gene at work. A mutant cell harboring this gene also became resistant against gamma-rays. The present results suggest that the protein in question is the Mn-SOD of R. radiotolerans, a good candidate as a radio-protection factor for this bacterial radio-resistance.
|
| 巻・号 |
52(6)
|
| ページ |
735-42
|
| 公開日 |
2011-1-1
|
| DOI |
10.1269/jrr.11105
|
| PII |
JST.JSTAGE/jrr/11105
|
| PMID |
21971036
|
| MeSH |
Actinobacteria / enzymology*
Actinobacteria / genetics
Actinobacteria / radiation effects*
Amino Acid Sequence
Bacterial Proteins / chemistry
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Base Sequence
DNA, Bacterial / genetics
Gamma Rays
Genes, Bacterial
Molecular Sequence Data
Protein Structure, Quaternary
Radiation Tolerance / genetics
Radiation Tolerance / physiology
Superoxide Dismutase / chemistry
Superoxide Dismutase / genetics
Superoxide Dismutase / metabolism*
|
| IF |
1.95
|
| 引用数 |
9
|
|
WOS 分野
|
BIOLOGY
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
|
| リソース情報 |
| 一般微生物 |
JCM 2153 |
