RRC ID 16001
著者 Hojo M, Ohkusa T, Tomeoku H, Koido S, Asaoka D, Nagahara A, Watanabe S.
タイトル Corticotropin-releasing factor secretion from dendritic cells stimulated by commensal bacteria.
ジャーナル World J Gastroenterol
Abstract AIM:To study the production and secretion of corticotropin-releasing factor (CRF) by dendritic cells and the influence of commensal bacteria.
METHODS:JAWSII cells (ATCC CRL-11904), a mouse dendritic cell line, were seeded into 24-well culture plates and grown for 3 d. Commensal bacterial strains of Clostridium clostrodiiforme (JCM1291), Bacteroides vulgatus (B. vulgatus) (JCM5856), Escherichia coli (JCM1649), or Fusobacterium varium (F. varium) (ATCC8501) were added to the cells except for the control well, and incubated for 2 h. After incubation, we performed enzyme-linked immunosorbent assay for the cultured medium and reverse transcription polymerase chain reaction for the dendritic cells, and compared these values with controls.
RESULTS:The level of CRF secretion by control dendritic cells was 40.4 ± 6.2 pg/mL. The CRF levels for cells incubated with F. varium and B. vulgatus were significantly higher than that of the control (P < 0.0001). CRF mRNA was present in the control sample without bacteria, and CRF mRNA levels in all samples treated with bacteria were above that of the control sample. F. varium caused the greatest increase in CRF mRNA expression.
CONCLUSION:Our results suggest that dendritic cells produce CRF, a process augmented by commensal bacteria.
巻・号 17(35)
ページ 4017-22
公開日 2011-9-21
DOI 10.3748/wjg.v17.i35.4017
PMID 22046091
PMC PMC3199561
MeSH Animals Bacteroides / metabolism* Cell Line Clostridium / metabolism* Corticotropin-Releasing Hormone / genetics Corticotropin-Releasing Hormone / metabolism* Dendritic Cells / cytology Dendritic Cells / metabolism* Dendritic Cells / microbiology* Escherichia coli / metabolism* Fusobacterium / metabolism* Mice RNA, Messenger / metabolism
IF 3.665
引用数 8
WOS 分野 GASTROENTEROLOGY & HEPATOLOGY
リソース情報
一般微生物 JCM 1219 JCM 1649 JCM 5826