| RRC ID |
16017
|
| 著者 |
Hasegawa A, Mochida K, Matoba S, Yonezawa K, Ohta A, Watanabe G, Taya K, Ogura A.
|
| タイトル |
Efficient production of offspring from Japanese wild-derived strains of mice (Mus musculus molossinus) by improved assisted reproductive technologies.
|
| ジャーナル |
Biol Reprod
|
| Abstract |
Because the genetic diversity of the laboratory mouse (Mus musculus) is very limited, wild-derived strains from this genus could provide invaluable experimental models for studies of mouse genetics and epigenetics such as quantitative trait locus analysis. However, such strains generally show poor reproductive performance under conventional husbandry conditions, so their use for large-scale analyses has been limited. This study was undertaken to devise assisted reproductive technologies (ARTs) for the efficient production of offspring in two wild-derived strains, MSM/Ms and JF1/Ms (Mus musculus molossinus). First, as females of these strains are poor responders to equine chorionic gonadotropin (eCG) stimulation, we examined the efficiency of superovulation by injecting anti-inhibin serum followed by human chorionic gonadotropin (hCG). Approximately four to six times more oocytes were ovulated than with eCG-hCG treatment in both strains, reaching ∼25-30 oocytes per female. Consequently, the procedures for in vitro fertilization using these superovulated oocytes and cryopreservation of embryos and spermatozoa could be optimized for both of the wild-derived strains. However, MSM/Ms embryos but not JF1/Ms embryos failed to develop to term after embryo transfer because of intrauterine death at mid to late gestation. We were able to overcome this obstacle by cotransfer of these embryos with those from laboratory strains combined with treatment of recipient females with an immunosuppressant (cyclosporin A). Thus, a series of ARTs essential for efficient production and preservation of the wild-derived strains were successfully devised. These technologies will facilitate systematic studies of mouse genetics and epigenetics using a wider range of genetic diversity than currently available in the genus Mus.
|
| 巻・号 |
86(5)
|
| ページ |
167, 1-7
|
| 公開日 |
2012-5-1
|
| DOI |
10.1095/biolreprod.111.098491
|
| PII |
biolreprod.111.098491
|
| PMID |
22337332
|
| MeSH |
Animals
Animals, Wild / genetics
Animals, Wild / physiology*
Chorionic Gonadotropin / therapeutic use
Cryopreservation / methods
Cyclosporine / therapeutic use
Female
Fertility / drug effects*
Fertility / physiology
Fertility Agents / therapeutic use*
Genetic Variation
Immunosuppressive Agents / therapeutic use
Inhibins / antagonists & inhibitors
Japan
Male
Mice / genetics
Mice / physiology*
Reproductive Techniques, Assisted*
Spermatozoa / drug effects
Spermatozoa / physiology
Superovulation / drug effects
Superovulation / physiology
|
| IF |
3.322
|
| 引用数 |
16
|
|
WOS 分野
|
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00209
RBRC00639 |
