論文 - 詳細
| RRC ID | 16020 |
|---|---|
| 著者 | Maday S, Wallace KE, Holzbaur EL. |
| タイトル | Autophagosomes initiate distally and mature during transport toward the cell soma in primary neurons. |
| ジャーナル | J Cell Biol |
| Abstract |
Autophagy is an essential cellular degradation pathway in neurons; defects in autophagy are sufficient to induce neurodegeneration. In this paper, we investigate autophagosome dynamics in primary dorsal root ganglion neurons. Autophagosome biogenesis occurs distally in a constitutive process at the neurite tip. Autophagosomes initially move bidirectionally and then switch to unidirectional, processive movement toward the cell soma driven by dynein. Autophagosomes copurify with anterograde and retrograde motors, suggesting that the activity of bound kinesin motors is effectively down-regulated to yield robust retrograde motility driven by dynein. Both organelle and soluble cargoes are internalized into autophagosomes, including mitochondria and ubiquitin. As autophagosomes move distally to proximally, they undergo maturation and become increasingly acidified, consistent with the formation of an autolysosomal compartment that may more efficiently degrade cargo. This maturation is accompanied by a switch to bidirectional motility characteristic of lysosomes. Together, autophagosome biogenesis and maturation in primary neurons is a constitutive process that is spatially and temporally regulated along the axon. |
| 巻・号 | 196(4) |
| ページ | 407-17 |
| 公開日 | 2012-2-20 |
| DOI | 10.1083/jcb.201106120 |
| PII | jcb.201106120 |
| PMID | 22331844 |
| PMC | PMC3283992 |
| MeSH | Animals Autophagy* Axons / physiology* Biological Transport Cell Compartmentation Dyneins / metabolism Fluorescent Antibody Technique Green Fluorescent Proteins / metabolism Image Processing, Computer-Assisted Mice Mice, Transgenic Neurons / cytology* Neurons / metabolism* Phagosomes / physiology* Superoxide Dismutase / physiology Superoxide Dismutase-1 |
| IF | 8.811 |
| 引用数 | 296 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |