論文 - 詳細
| RRC ID | 16021 |
|---|---|
| 著者 | Tsuruyama T, Liu W, Yoshikawa K. |
| タイトル | In vitro murine leukemia retroviral integration and structure fluctuation of target DNA. |
| ジャーナル | PLoS One |
| Abstract |
Integration of the retroviral genome into host DNA is a critical step in the life cycle of a retrovirus. Although assays for in vitro integration have been developed, the actual DNA sequences targeted by murine leukemia retrovirus (MLV) during in vitro reproduction are unknown. While previous studies used artificial target sequences, we developed an assay using target DNA sequences from common MLV integration sites in Stat5a and c-myc in the genome of murine lymphomas and successfully integrated MLV into the target DNA in vitro. We calculated the free energy change during folding of the target sequence DNA and found a close correlation between the calculated free energy change and the number of integrations. Indeed, the integrations closely correlated with fluctuation of the structure of the target DNA segment. These data suggest that the fluctuation may generate a DNA structure favorable for in vitro integration into the target DNA. The approach described here can provide data on the biochemical properties of the integration reaction to which the target DNA structure may contribute. |
| 巻・号 | 7(2) |
| ページ | e31533 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0031533 |
| PII | PONE-D-11-21298 |
| PMID | 22348097 |
| PMC | PMC3279379 |
| MeSH | Animals Base Sequence DNA, Viral / chemistry* Gene Targeting* Genome, Viral Leukemia Virus, Murine / genetics* Mice Nucleic Acid Conformation Proto-Oncogene Proteins c-myc / genetics Retroviridae / genetics STAT5 Transcription Factor / genetics Thermodynamics Virus Integration* |
| IF | 2.74 |
| 引用数 | 4 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 実験動物マウス | RBRC00222 |