論文 - 詳細
| RRC ID | 16028 |
|---|---|
| 著者 | Lee M, Woo SG, Ten LN. |
| タイトル | Shinella daejeonensis sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A Gram-negative-staining, motile, rod-shaped, aerobic bacterial strain, designated MJ02(T), was isolated from sludge of a leachate treatment plant in Daejeon (South Korea) and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ02(T) belonged to the family Rhizobiaceae, class Alphaproteobacteria, and was most closely related to Shinella yambaruensis MS4(T) (97.6 % sequence similarity) and Shinella fusca DC-196(T) (97.5 %). The G+C content of the genomic DNA of strain MJ02(T) was 64.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone and a fatty acid profile with C₁₈:₁ω7c (45.8 %) and C₁₆:₀ (21.8 %) as the major components supported the affiliation of strain MJ02(T) to the genus Shinella. However, strain MJ02(T) exhibited relatively low levels of DNA-DNA relatedness with respect to S. fusca DSM 21319(T) (17±7 %) and S. yambaruensis KACC 14483(T) (12±6 %), showing clearly that the isolate constituted a new genospecies. Strain MJ02(T) could be clearly differentiated from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ02(T) is considered to represent a novel species of the genus Shinella, for which the name Shinella daejeonensis sp. nov. is proposed. The type strain is MJ02(T) ( = KCTC 22450(T) = JCM 16236(T)). |
| 巻・号 | 61(Pt 9) |
| ページ | 2123-2128 |
| 公開日 | 2011-9-1 |
| DOI | 10.1099/ijs.0.026435-0 |
| PMID | 20870886 |
| MeSH | Bacterial Typing Techniques Base Composition Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Korea Locomotion Molecular Sequence Data Nitrates / metabolism* Nucleic Acid Hybridization Oxidation-Reduction Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Rhizobiaceae / classification* Rhizobiaceae / genetics Rhizobiaceae / isolation & purification* Rhizobiaceae / physiology Sequence Analysis, DNA Sewage / microbiology* |
| IF | 2.415 |
| 引用数 | 11 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 16236 |