論文 - 詳細
| RRC ID | 16030 |
|---|---|
| 著者 | Balboa S, Doce A, Diéguez AL, Romalde JL. |
| タイトル | Evaluation of different species-specific PCR protocols for the detection of Vibrio tapetis. |
| ジャーナル | J Invertebr Pathol |
| Abstract |
In this study the specificity and sensitivity of three primer pairs, Jvt1-Jvt2, VtF-VtR and VtKF-VtKR, for the detection of Vibrio tapetis were evaluated in parallel using 23 V. tapetis strains isolated from different mollusc and fish species and with different geographical origin, as well as 29 representatives of related Vibrio species. The three primer pairs amplified all the V. tapetis strains, regardless of their host or geographical origin. However, with primer sets VtF-VtR and VtKF-VtKR amplification products of the expected size were obtained from chromosomal DNA of some of the non-V. tapetis bacteria tested. The sensitivity of the three PCR detection methods was also different. The detection limit obtained with primer pairs Jvt1-Jvt2 and VtF-VtR was between 1 and 10 pg DNA/PCR tube (2-20 bacterial cells per reaction). The primer set VtKF-VtKR showed a reduction of sensitivity in at least one order of magnitude. The results were highly reproducible with all primer sets when using the same thermal cycler, although some differences were observed in the results obtained in different PCR machines. Based on the findings reported here, we propose the Jvt1-Jvt2 PCR protocol as the most adequate for an accurate detection of V. tapetis in diagnostic pathology as well as in epidemiological studies of this clam pathogen. |
| 巻・号 | 108(2) |
| ページ | 85-91 |
| 公開日 | 2011-10-1 |
| DOI | 10.1016/j.jip.2011.06.010 |
| PII | S0022-2011(11)00116-9 |
| PMID | 21771598 |
| MeSH | Animals DNA, Bacterial / isolation & purification Fish Diseases / diagnosis Fish Diseases / microbiology Fishes / microbiology Limit of Detection Mollusca / microbiology Polymerase Chain Reaction / methods* Predictive Value of Tests Species Specificity Vibrio / genetics Vibrio / isolation & purification* Vibrio Infections / diagnosis* Vibrio Infections / microbiology |
| IF | 2.074 |
| 引用数 | 8 |
| WOS 分野 | ZOOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 21271 |