RRC ID |
16064
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著者 |
Mon H, Izumi M, Mitsunobu H, Tatsuke T, Iiyama K, Jikuya H, Lee JM, Kusakabe T.
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タイトル |
Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori.
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ジャーナル |
Insect Biochem Mol Biol
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Abstract |
Epigenetic information is encoded in post-translational modifications (PTMs) of histones. Various combinations of these marks contribute to the regulation of chromatin-templated DNA metabolisms. The histone code is gradually translated into biological responses in model organisms. However, in the silkworm, the modifications of histones with unique holocentric chromosomes have not yet been analyzed. TAU-PAGE analysis of the silkworm histone variants H2A, H2B, and H3, separated by RP-HPLC, suggested silkworm specific modification. Detailed mass spectrometry analyses of the peptides derived from the N-terminus of the silkworm H3.2 generated by glutamyl endopeptidase, lysyl endopeptidase, and trypsin digestions revealed global modifications around H3K9.
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巻・号 |
41(11)
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ページ |
902-8
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公開日 |
2011-11-1
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DOI |
10.1016/j.ibmb.2011.08.004
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PII |
S0965-1748(11)00162-7
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PMID |
21914479
|
MeSH |
Animals
Bombyx / chemistry
Bombyx / genetics
Bombyx / metabolism*
Cell Line
Chromatography, High Pressure Liquid
Histones / chemistry
Histones / isolation & purification
Histones / metabolism*
Mass Spectrometry
Protein Processing, Post-Translational*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
IF |
3.827
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引用数 |
3
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WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
カイコ |
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