RRC ID 1624
著者 Higasa K, Hayashi K.
タイトル Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing.
ジャーナル Nucleic Acids Res
Abstract We describe a method for the efficient genotyping of SNPs, involving sequencing of ordered and catenated sequence-tagged sites (OCS). In OCS, short genomic segments, each containing an SNP, are amplified by PCR using primers that carry specially designed extra nucleotides at their 5'-ends. Amplification products are then combined and converted to a concatamer in a defined order by a second round of thermal cycling. The concatenation takes place because the 5'-ends of each amplicon are designed to be complementary to the ends of the presumptive neighboring amplicons. The primer sequences for OCS are chosen using newly developed dedicated software, OCS Optimizer. Using sets of SNPs, we show that at least 10 STSs can be concatenated in a predefined order and all SNPs in the STSs are accurately genotyped by one two-way sequencing reaction.
巻・号 30(3)
ページ E11
公開日 2002-2-1
DOI 10.1093/nar/30.3.e11
PMID 11809899
PMC PMC100310
MeSH Asians / genetics Base Sequence DNA / analysis DNA / chemistry DNA / genetics DNA Primers / genetics Gene Order / genetics Genotype Heterozygote Humans Japan Molecular Sequence Data Nucleic Acid Conformation Polymerase Chain Reaction / methods Polymorphism, Single Nucleotide / genetics* Sensitivity and Specificity Sequence Alignment Sequence Analysis, DNA / methods* Sequence Tagged Sites* Software
IF 11.502
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
情報