RRC ID 1667
Author Manna D, Breier AM, Higgins NP.
Title Microarray analysis of transposition targets in Escherichia coli: the impact of transcription.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract 1,000-fold variation in utilization frequency. A moderate preference was observed for genes near the origin compared to terminus of replication. Large biases were found at hot and cold spots, which often include several consecutive genes. Efficient transcription of genes had a strong negative influence on transposition. Our results indicate that local chromosome structure is more important than DNA sequence in determining Mu target-site selection.
Volume 101(26)
Pages 9780-5
Published 2004-6-29
DOI 10.1073/pnas.0400745101
PII 0400745101
PMID 15210965
PMC PMC470751
MeSH Bacteriophage mu / genetics* DNA Transposable Elements / genetics* DNA, Viral / genetics Escherichia coli / genetics* Escherichia coli / virology Gene Expression Regulation, Bacterial Genes, Bacterial / genetics* Genome, Bacterial Multigene Family / genetics Mutagenesis / genetics Oligonucleotide Array Sequence Analysis* Replication Origin / genetics Transcription, Genetic / genetics* Virus Integration / genetics*
IF 9.504
Times Cited 28