RRC ID 1716
著者 Cardoso JC, Clark MS, Viera FA, Bridge PD, Gilles A, Power DM.
タイトル The secretin G-protein-coupled receptor family: teleost receptors.
ジャーナル J Mol Endocrinol
Abstract Twenty-one members of the secretin family (family 2) of G-protein-coupled receptors (GPCRs) were identified via directed cloning and data-mining of the Fugu Genome Consortium database, representing the most comprehensive description of secretin GPCRs in a teleost fish to date. Duplicated genes were identified for many of the family members, namely the receptors for pituitary adenylate cyclase-activating polypeptide (PACAP)/vasoactive intestinal peptide (VIP), calcitonin, calcitonin gene-related peptide (CGRP), growth hormone releasing hormone (GHRH), glucagon receptor/glucagon-like peptide (GLP) and parathyroid hormone-related peptide (PTHrP)/PTH. Mining of other teleost genomes (zebrafish and Tetraodon) revealed that the duplicated genes identified in the Takifugu genome were also present in these fish. Additional database searching of the Escherichia coli, yeast, Drosophila, Caenorhabditis elegans and Ciona genomes revealed that the family 2 of GPCRs were only present in the multicellular organisms. Orthologues of all the human secretin receptors were identified with the exception of secretin itself. Additional database searches in the Fugu Genome Consortium database also failed to reveal a secretin ligand and so it is hypothesised that both the receptor and the ligand evolved after the divergence of teleost/tetrapod lineages. Phylogenetic analysis at both the protein and the DNA level provided strong support for each of the individual receptor family groupings, but weak support between groups, making evolutionary inferences difficult. A more critical analysis of the PACAP/VIP receptor family confirmed previous hypotheses that the vasoactive intestinal peptide receptor (VPAC(1)R) gene is the ancestral form of the receptor.
巻・号 34(3)
ページ 753-65
公開日 2005-6-1
DOI 10.1677/jme.1.01730
PII 34/3/753
PMID 15956345
MeSH Animals Calcitonin / metabolism Calcitonin Gene-Related Peptide / metabolism Fishes Growth Hormone-Releasing Hormone / metabolism Humans Nerve Growth Factors / metabolism Neuropeptides / metabolism Neurotransmitter Agents / metabolism Parathyroid Hormone / metabolism Parathyroid Hormone-Related Protein / metabolism Pituitary Adenylate Cyclase-Activating Polypeptide Receptors, G-Protein-Coupled / metabolism* Receptors, Vasoactive Intestinal Peptide Secretin / metabolism* Vasoactive Intestinal Peptide / metabolism Zebrafish Proteins
IF 3.562
引用数 29
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
情報 Medaka