RRC ID 1722
Author Jung KH, Han MJ, Lee YS, Kim YW, Hwang I, Kim MJ, Kim YK, Nahm BH, An G.
Title Rice Undeveloped Tapetum1 is a major regulator of early tapetum development.
Journal Plant Cell
Abstract The tapetum, the innermost of four sporophytic layers in the anther wall, comes in direct contact with the developing male gametophyte and is thought to play a crucial role in the development and maturation of microspores. Here, we report the identification of rice (Oryza sativa) Undeveloped Tapetum1 (Udt1), which is required for the differentiation of secondary parietal cells to mature tapetal cells. T-DNA or retrotransposon Tos17 insertions in the Udt1 gene caused male sterility. The anther walls and meiocytes of the mutants were normal during the early premeiosis stage, but their tapeta failed to differentiate and became vacuolated during the meiotic stage. In addition, meiocytes did not develop to microspores, and middle layer degeneration was inhibited. Consequently, the anther locules contained no pollen. The UDT1:green fluorescent protein fusion protein was localized to the nucleus. This, together with its homology with other basic helix-loop-helix proteins, suggests that UDT1 is a transcription factor. DNA microarray analysis identified 958 downregulated and 267 upregulated genes in the udt1-1 anthers, suggesting that Udt1 plays a major role in maintaining tapetum development, starting in early meiosis.
Volume 17(10)
Pages 2705-22
Published 2005-10
DOI 10.1105/tpc.105.034090
PII tpc.105.034090
PMID 16141453
PMC PMC1242267
MeSH Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / genetics Cell Differentiation / genetics DNA, Bacterial / genetics Flowers / genetics Flowers / growth & development* Flowers / metabolism Gene Expression Regulation, Plant / genetics Genes, Plant / genetics Germ Cells / growth & development Germ Cells / metabolism Meiosis / genetics Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism* Oryza / genetics Oryza / growth & development* Oryza / metabolism Plant Infertility / genetics Plant Proteins / genetics Plant Proteins / metabolism* Regulatory Elements, Transcriptional / genetics Retroelements / genetics Sequence Homology, Amino Acid Spores / genetics Spores / growth & development Spores / metabolism Transcription Factors / genetics Transcription Factors / metabolism*
IF 8.228
Times Cited 159
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
INFORMATION Rice