| RRC ID |
17396
|
| 著者 |
Shiraishi H, Itoh T, Hayashi H, Takagi K, Sakane M, Mori T, Wang J.
|
| タイトル |
Electrochemical detection of E. coli 16S rDNA sequence using air-plasma-activated fullerene-impregnated screen printed electrodes.
|
| ジャーナル |
Bioelectrochemistry
|
| Abstract |
A new DNA modified electrode for the electrochemical detection of 16S rDNA extracted from Escherichia coli (JCM1649) is proposed. The electrodes were fabricated by screen printing a fullerene-impregnated carbon ink onto a poly(methylmethacrylate) substrate and immobilizing a probe DNA on the surface after activating the electrode with air plasma. The results indicated a dramatic improvement in the surface coverage of the immobilized probe DNA, and of the reduction peak of the redox indicator (Co(phen)(3)(3+)) due to the incorporation of fullerene. By immobilizing the probe onto the fullerene-impregnated screen-printed electrodes, the PCR product of the 16S rDNA extracted from E. coli was directly detected without any pretreatment. A well defined signal difference was observed between the perfectly matching oligonucleotide and the mismatching one, and it was possible to detect the target at the modified electrode. This method enabled us to clearly detect the two base mismatches in the ca. 1500-bases long 16S rDNA sequence.
|
| 巻・号 |
70(2)
|
| ページ |
481-7
|
| 公開日 |
2007-5-1
|
| DOI |
10.1016/j.bioelechem.2006.07.011
|
| PII |
S1567-5394(06)00101-0
|
| PMID |
16962387
|
| MeSH |
Air
Base Pair Mismatch
DNA, Bacterial / chemistry
DNA, Bacterial / genetics*
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics*
Electrochemistry / instrumentation
Electrochemistry / methods*
Electrodes
Escherichia coli / genetics*
Fullerenes / chemistry
Hot Temperature
In Situ Hybridization / instrumentation
In Situ Hybridization / methods*
RNA, Ribosomal, 16S / chemistry
RNA, Ribosomal, 16S / genetics*
Sequence Analysis, DNA / methods*
Surface Properties
|
| IF |
4.722
|
| 引用数 |
39
|
|
WOS 分野
|
BIOLOGY
BIOPHYSICS
ELECTROCHEMISTRY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1649 |
