論文 - 詳細
| RRC ID | 17412 |
|---|---|
| 著者 | Sakai T, Yuasa K, Ozaki A, Sano M, Okuda R, Nakai T, Iida T. |
| タイトル | Genotyping of Edwardsiella ictaluri isolates in Japan using amplified-fragment length polymorphism analysis. |
| ジャーナル | Lett Appl Microbiol |
| Abstract |
AIM:The major objective of the present study was to clarify genetic relationship of isolates of Edwardsiella ictaluri in Japan, which was first found from ayu Plecoglossus altivelis in Japanese rivers in 2007. METHODS AND RESULTS:Ten isolates of Edw. ictaluri in 2007-2008 from ayu and the 1 isolate from bagrid catfish Pelteobagrus nudiceps in Japan were subjected to amplified-fragment length polymorphism (AFLP) analysis. The strains isolated from catfish in United States (ATCC strains) or Indonesia were used as reference strains. The AFLP profiles were all the same among the isolates from Japan, while the polymorphic DNA bands were observed among the strains from United States or Indonesia. The isolates from Japan and Indonesia constituted a genogroup different from the ATCC strains on a dendrogram constructed from the AFLP profiles. CONCLUSION:No DNA polymorphisms were found among Japanese Edw. ictaluri isolates. SIGNIFICANCE AND IMPACT OF THE STUDY:A single clonality of the Edw. ictaluri isolates in Japan suggests the single source of the organism, and the infection in ayu is in the early stage of epidemics. |
| 巻・号 | 49(4) |
| ページ | 443-9 |
| 公開日 | 2009-10-1 |
| DOI | 10.1111/j.1472-765X.2009.02686.x |
| PII | LAM2686 |
| PMID | 19674293 |
| MeSH | Amplified Fragment Length Polymorphism Analysis Animals Bacterial Typing Techniques Catfishes / microbiology* DNA, Bacterial / genetics Edwardsiella ictaluri / classification Edwardsiella ictaluri / genetics* Edwardsiella ictaluri / isolation & purification Enterobacteriaceae Infections / microbiology Enterobacteriaceae Infections / veterinary* Fish Diseases / microbiology* Genotype Japan Osmeriformes / microbiology* Phylogeny United States |
| IF | 2.173 |
| 引用数 | 8 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1680 |