| RRC ID |
17417
|
| Author |
Ito K, Ito S, Shimamura T, Kawarasaki Y, Abe K, Misaka T, Kobayashi T, Iwata S.
|
| Title |
Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.
|
| Journal |
Acta Crystallogr Sect F Struct Biol Cryst Commun
|
| Abstract |
Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF-SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Orthorhombic GTF-SI crystals belonging to space group P2(1)2(1)2 were obtained. A diffraction data set was collected to 2.1 A resolution.
|
| Volume |
66(Pt 9)
|
| Pages |
1086-8
|
| Published |
2010-9-1
|
| DOI |
10.1107/S1744309110029714
|
| PII |
S1744309110029714
|
| PMID |
20823533
|
| PMC |
PMC2935234
|
| MeSH |
Crystallization
Crystallography, X-Ray
Glycosyltransferases / chemistry*
Streptococcus mutans / enzymology*
|
| IF |
0.968
|
| Times Cited |
5
|
|
WOS Category
|
CRYSTALLOGRAPHY
BIOCHEMICAL RESEARCH METHODS
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 5175 |
