論文 - 詳細
| RRC ID | 17419 |
|---|---|
| 著者 | Yoshida Y, Ito S, Kamo M, Kezuka Y, Tamura H, Kunimatsu K, Kato H. |
| タイトル | Production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586. |
| ジャーナル | Microbiology (Reading) |
| Abstract |
Fusobacterium nucleatum produces a large amount of the toxic metabolite hydrogen sulfide in the oral cavity. Here, we report the molecular basis of F. nucleatum H(2)S production, which is associated with two different enzymes: the previously reported Cdl (Fn1220) and the newly identified Lcd (Fn0625). SDS-PAGE analysis with activity staining revealed that crude enzyme extracts from F. nucleatum ATCC 25586 contained three major H(2)S-producing proteins. Two of the proteins with low molecular masses migrated similarly to purified Fn0625 and Fn1220. Their kinetic values suggested that Fn0625 had a lower enzymic capacity to produce H(2)S from L-cysteine (approximately 30%) than Fn1220. The Fn0625 protein degraded a variety of substrates containing betaC-S linkages to produce ammonia, pyruvate and sulfur-containing products. Unlike Fn0625, Fn1220 produced neither pyruvate nor ammonia from L-cysteine. Reversed-phase HPLC separation and mass spectrometry showed that incubation of L-cysteine with Fn1220 produced H(2)S and an uncommon amino acid, lanthionine, which is a natural constituent of the peptidoglycans of F. nucleatum ATCC 25586. In contrast, most of the sulfur-containing substrates tested, except L-cysteine, were not used by Fn1220. Real-time PCR analysis demonstrated that the fn1220 gene showed several-fold higher expression than fn0625 and housekeeping genes in exponential-phase cultures of F. nucleatum. Thus, we conclude that Fn0625 and Fn1220 produce H(2)S in distinct manners: Fn0625 carries out beta-elimination of L-cysteine to produce H(2)S, pyruvate and ammonia, whereas Fn1220 catalyses the beta-replacement of L-cysteine to produce H(2)S and lanthionine, the latter of which may be used for peptidoglycan formation in F. nucleatum. |
| 巻・号 | 156(Pt 7) |
| ページ | 2260-2269 |
| 公開日 | 2010-7-1 |
| DOI | 10.1099/mic.0.039180-0 |
| PMID | 20413556 |
| MeSH | Alanine / analogs & derivatives* Alanine / biosynthesis Ammonia / metabolism Aspartate Aminotransferases / chemistry Aspartate Aminotransferases / genetics Aspartate Aminotransferases / metabolism* Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cysteine Synthase / chemistry Cysteine Synthase / genetics Cysteine Synthase / metabolism* Fusobacterium nucleatum / chemistry Fusobacterium nucleatum / enzymology Fusobacterium nucleatum / genetics Fusobacterium nucleatum / metabolism* Homocysteine / biosynthesis* Hydrogen Sulfide / metabolism* Kinetics Pyruvic Acid / metabolism Substrate Specificity Sulfides |
| IF | 2.138 |
| 引用数 | 23 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 8532 JCM 12990 |