| RRC ID |
17422
|
| Author |
Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, Lee KM, Kawabata M, Shirakawa T.
|
| Title |
Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model.
|
| Journal |
Vaccine
|
| Abstract |
We developed a novel vaccine platform utilizing Bifidobacterium as an antigen delivery vehicle for mucosal immunization. Genetically modified Bifidobacterium longum displaying Salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. The efficiency of this vaccine was evaluated in a murine model of typhoid fever. We then orally administered 2.5 × 10(7) CFU of the recombinant Bifidobacterium longum (vaccine) or parental Bifidobacterium longum, or PBS to BALB/C mice every other day for 2 weeks. After the administration, a total of 42 mice (14 mice in each group) were challenged with Salmonella Typhimurium (1.0 × 10(7) CFU/mouse). While 12 mice in the PBS group, and 9 in the parental Bifidobacterium longum group died (median survival: 14 and 25 days), only two in the vaccine group died. These data support that our genetically modified Bifidobacterium antigen delivery system offers a promising vaccine platform for inducing efficient mucosal immunity.
|
| Volume |
28(41)
|
| Pages |
6684-91
|
| Published |
2010-9-24
|
| DOI |
10.1016/j.vaccine.2010.08.007
|
| PII |
S0264-410X(10)01116-3
|
| PMID |
20709009
|
| MeSH |
Animals
Antibodies, Bacterial / immunology
Antigens, Bacterial / immunology
Bifidobacterium / immunology*
Disease Models, Animal
Female
Flagellin / immunology
Immunity, Mucosal*
Mice
Mice, Inbred BALB C
Recombinant Fusion Proteins / immunology
Salmonella typhimurium / immunology
Typhoid Fever / immunology
Typhoid Fever / prevention & control*
Typhoid-Paratyphoid Vaccines / immunology*
|
| IF |
3.143
|
| Times Cited |
21
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
|
| Resource |
| General Microbes |
JCM 1217 |
