| RRC ID |
17429
|
| 著者 |
Tohno M, Shimosato T, Aso H, Kitazawa H.
|
| タイトル |
Immunobiotic Lactobacillus strains augment NLRP3 expression in newborn and adult porcine gut-associated lymphoid tissues.
|
| ジャーナル |
Vet Immunol Immunopathol
|
| Abstract |
We isolated cDNA encoding porcine nucleotide-binding domain-like receptor family, pryin domain containing 3 (NLRP3) from Peyer's patches. The complete nucleotide open reading frame of porcine NLRP3 contains 3108-bp encoding a deduced polypeptide of 1036-amino acid residues. The porcine NLRP3 amino acid sequence is more similar to the longest isoform of human than the mouse counterpart. The predicted amino acid sequence of porcine NLRP3 presented nine C-terminal leucine-rich repeat domains. In newborn swine, the expression of NLRP3 was detected at higher levels in spleen and mesenteric lymph nodes, while lower levels were observed in intestinal tissues. In adult swine, NLRP3 was strongly expressed in Peyer's patches and the mesenteric lymph nodes, and the expression level in the lower intestinal tissues was comparable to that in spleen. Toll-like receptor and nucleotide-binding domain ligands, as well as Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus gasseri, enhanced NLRP3 expression in gut-associated lymphoid tissues (GALT) of newborn and adult swine. Our results should aid in understanding the intestinal immunoregulatory mechanisms underlying NLRP3 activation and the priming ability of immunobiotic lactic acid bacteria in porcine GALT.
|
| 巻・号 |
144(3-4)
|
| ページ |
410-6
|
| 公開日 |
2011-12-15
|
| DOI |
10.1016/j.vetimm.2011.09.010
|
| PII |
S0165-2427(11)00366-7
|
| PMID |
22024502
|
| MeSH |
Amino Acid Sequence
Animals
Animals, Newborn / immunology
Animals, Newborn / metabolism
Animals, Newborn / microbiology
Base Sequence
Carrier Proteins / genetics
Carrier Proteins / immunology
Carrier Proteins / metabolism*
Cloning, Molecular
Gene Expression Regulation / immunology
Humans
Inflammasomes / genetics
Inflammasomes / immunology
Inflammasomes / metabolism
Lactobacillus / immunology*
Lactobacillus delbrueckii / immunology
Lymph Nodes / metabolism
Mice
Molecular Sequence Data
NLR Family, Pyrin Domain-Containing 3 Protein
Nucleotides / metabolism
Peyer's Patches / immunology
Peyer's Patches / metabolism*
Probiotics / metabolism
Sequence Analysis, Protein
Spleen / metabolism
Swine / immunology*
Swine / microbiology
|
| IF |
1.713
|
| 引用数 |
16
|
|
WOS 分野
|
VETERINARY SCIENCES
IMMUNOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1131 |
