| RRC ID |
17434
|
| 著者 |
Zhang J, van Hung P, Hayashi M, Yoshida S, Ohkusu K, Ezaki T.
|
| タイトル |
DnaJ sequences of Bacillus cereus strains isolated from outbreaks of hospital infection are highly similar to Bacillus anthracis.
|
| ジャーナル |
Diagn Microbiol Infect Dis
|
| Abstract |
Bacillus cereus is becoming an important nomosomial pathogen because of frequent isolation from blood cultures and from severe systemic infections. To differentiate highly pathogenic outbreak strain of B. cereus from other sources of the Bacillus cereus, we attempted to analyze their dnaJ sequences. Assays indicated that dnaJ sequence similarity of all of 52 blood culture isolates of B. cereus ranged from 92.8% to 100%. The distance between B. anthracis and B. cereus except six outbreak isolates ranged from 3.8% to 6.4%. The dnaJ sequences of six outbreak strains of B. cereus (GTC 02891, GTC 02896, GTC 02916, GTC 02917, GTC 03221, and GTC 03222) were closely related to those of B. anthracis (99.2%-99.5% sequence similarity). Ba813 sequences were only found in the six outbreak strains of B. cereus. The other pathogenic factors of B. anthracis were not found in these six outbreak strains, with the exception of GTC 02891 (cap-positive). The six outbreak strains formed clear β-hemolytic colonies on a sheep blood agar plate. Our findings suggest that outbreak strains of B. cereus isolated from blood cultures are likely to have the risk of causing serious infection, and dnaJ and Ba813 are important markers to identify such strains. Phylogenetic analysis of dnaJ and MLST revealed that the six outbreak strains of B. cereus are closely related to B. anthracis.
|
| 巻・号 |
70(3)
|
| ページ |
307-15
|
| 公開日 |
2011-7-1
|
| DOI |
10.1016/j.diagmicrobio.2011.02.012
|
| PII |
S0732-8893(11)00095-2
|
| PMID |
21683265
|
| MeSH |
Bacillus anthracis / genetics*
Bacillus cereus / classification
Bacillus cereus / genetics*
Bacillus cereus / isolation & purification*
Bacteremia / epidemiology
Bacteremia / microbiology
Cluster Analysis
Cross Infection / epidemiology*
Cross Infection / microbiology
Culture Media / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Disease Outbreaks*
Gram-Positive Bacterial Infections / epidemiology*
Gram-Positive Bacterial Infections / microbiology
HSP40 Heat-Shock Proteins / genetics*
Hemolysis
Humans
Molecular Sequence Data
Multilocus Sequence Typing
Phylogeny
Sequence Analysis, DNA
|
| IF |
2.499
|
| 引用数 |
11
|
|
WOS 分野
|
INFECTIOUS DISEASES
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 2152 |
