| RRC ID |
17438
|
| Author |
Kimura M, Tanikawa T, Suzuki M, Koizumi N, Kamiyama T, Imaoka K, Yamada A.
|
| Title |
Detection of Streptobacillus spp. in feral rats by specific polymerase chain reaction.
|
| Journal |
Microbiol Immunol
|
| Abstract |
Streptobacillus moniliformis is an etiological agent of rat-bite fever and Haverhill fever in human infection. As the currently available methods for identifying the causative bacteria are not satisfactory, we attempted to establish them by PCR using newly designed primers for the 16S rRNA gene of S. moniliformis. We then determined the prevalence of Streptobacillus spp. in two species of feral rats that inhabit an urban region in Japan, because information on the prevalence of the bacteria in feral rats is obscure. The use of PCR with newly designed primers showed that an extremely high proportion of R. norvegicus harbored the bacteria (61/66, 92%), whereas the prevalence was only 58% in R. rattus (30/52). The nucleotide sequence analysis of the 16S rRNA gene of Streptobacillus spp. isolated from oral swabs of feral rats showed at least two different types of bacteria among isolates from R. norvegicus and R. rattus.
|
| Volume |
52(1)
|
| Pages |
9-15
|
| Published |
2008-1-1
|
| DOI |
10.1111/j.1348-0421.2008.00005.x
|
| PII |
MIM005
|
| PMID |
18352907
|
| MeSH |
Animals
Bacterial Typing Techniques
Cluster Analysis
DNA Primers / genetics
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fusobacterium Infections / microbiology
Japan
Molecular Sequence Data
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
Rats
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Streptobacillus / classification
Streptobacillus / isolation & purification*
|
| IF |
1.566
|
| Times Cited |
22
|
|
WOS Category
|
IMMUNOLOGY
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 3718
JCM 3721
JCM 11174
JCM 12969
JCM 20682 |
