論文 - 詳細
| RRC ID | 17442 |
|---|---|
| 著者 | Yoneyama F, Fukao M, Zendo T, Nakayama J, Sonomoto K. |
| タイトル | Biosynthetic characterization and biochemical features of the third natural nisin variant, nisin Q, produced by Lactococcus lactis 61-14. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:To characterize the genetic and biochemical features of nisin Q. METHODS AND RESULTS:The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster were identified. Nisin Q production was confirmed from the nisQ-introduced nisin Z producer. In the reporter assay, nisin Q exhibited an induction level that was threefold lower than that of nisin A. Nisin Q demonstrated an antimicrobial spectrum similar to those of the other nisins. Under oxidizing conditions, nisin Q retained a higher level of activity than nisin A. This higher oxidative tolerance could be attributed to the presence of only one methionine residue in nisin Q, in contrast to other nisins that contain two. CONCLUSIONS:The 11 orfs of the nisin producers were identical with regard to their functions. The antimicrobial spectra of the three natural nisins were similar. Nisin Q demonstrated higher oxidative tolerance than nisin A. SIGNIFICANCE AND IMPACT OF THE STUDY:Genetic and biochemical features of nisin Q are similar to those of other variants. Moreover, owing to its higher oxidative tolerance, nisin Q is a potential alternative for nisin A. |
| 巻・号 | 105(6) |
| ページ | 1982-90 |
| 公開日 | 2008-12-1 |
| DOI | 10.1111/j.1365-2672.2008.03958.x |
| PII | JAM3958 |
| PMID | 19120645 |
| MeSH | Anti-Bacterial Agents / biosynthesis* Anti-Bacterial Agents / pharmacology Bacteria / drug effects Bacteriocins / genetics* Bacteriocins / metabolism Bacteriocins / pharmacology DNA, Bacterial / genetics Lactococcus lactis / genetics* Lactococcus lactis / metabolism Microbial Sensitivity Tests Molecular Sequence Data Multigene Family / genetics Nisin / analogs & derivatives* Nisin / biosynthesis Nisin / genetics Nisin / pharmacology Open Reading Frames |
| IF | 3.066 |
| 引用数 | 17 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 7638 |