論文 - 詳細
| RRC ID | 17443 |
|---|---|
| 著者 | Oshiro M, Shinto H, Tashiro Y, Miwa N, Sekiguchi T, Okamoto M, Ishizaki A, Sonomoto K. |
| タイトル | Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
We proposed a kinetic simulation model of xylose metabolism in Lactococcus lactis IO-1 that describes the dynamic behavior of metabolites using the simulator WinBEST-KIT. This model was developed by comparing the experimental time-course data of metabolites in batch cultures grown in media with initial xylose concentrations of 20.3-57.8 g/l with corresponding calculated data. By introducing the terms of substrate activation, substrate inhibition, and product inhibition, the revised model showed a squared correlation coefficient (r2) of 0.929 between the experimental time-course of metabolites and the calculated data. Thus, the revised model is assumed to be one of the best candidates for kinetic simulation describing the dynamic behavior of metabolites. Sensitivity analysis revealed that pyruvate flux distribution is important for higher lactate production. To confirm the validity of our kinetic model, the results of the sensitivity analysis were compared with enzyme activities observed during increasing lactate production by adding natural rubber serum powder to the xylose medium. The experimental results on pyruvate flux distribution were consistent with the prediction by sensitivity analysis. |
| 巻・号 | 108(5) |
| ページ | 376-84 |
| 公開日 | 2009-11-1 |
| DOI | 10.1016/j.jbiosc.2009.05.003 |
| PII | S1389-1723(09)00245-X |
| PMID | 19804860 |
| MeSH | Bacterial Proteins / metabolism* Computer Simulation Kinetics Lactococcus lactis / classification Lactococcus lactis / metabolism* Metabolic Clearance Rate Models, Biological* Pyruvic Acid / metabolism* Signal Transduction / physiology* Software* Species Specificity Xylose / metabolism* |
| IF | 2.366 |
| 引用数 | 18 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 7638 |