論文 - 詳細
| RRC ID | 17447 |
|---|---|
| 著者 | Odamaki T, Yonezawa S, Kitahara M, Sugahara Y, Xiao JZ, Yaeshima T, Iwatsuki K, Ohkuma M. |
| タイトル | Novel multiplex polymerase chain reaction primer set for identification of Lactococcus species. |
| ジャーナル | Lett Appl Microbiol |
| Abstract |
AIMS:The gram-positive bacterial genus Lactococcus has been taxonomically classified into seven species (Lactococcus lactis, Lactococcus garvieae, Lactococcus piscium, Lactococcus plantarum, Lactococcus raffinolactis, Lactococcus chungangensis and Lactococcus fujiensis). This study aimed to develop a novel multiplex polymerase chain reaction (PCR) primer set for the identification of the seven lactococcal species, as well as to differentiate the two industrially important dairy subspecies, L. lactis subsp. lactis and L. lactis subsp. cremoris. METHODS AND RESULTS:A multiplex PCR primer set was designed based on the nucleotide sequences of the 16S rRNA gene of the seven lactococcal species. The specificity of the established one-step multiplex PCR scheme was verified using more than 200 bacterial strains, in which a complete sequence match was confirmed by partial sequencing of their 16S rRNA gene. CONCLUSIONS:The one-step multiplex PCR enables the identification and speciation of bacterial strains belonging to the genus Lactococcus and the differentiation of strains of L. lactis subsp. lactis and L. lactis subsp. cremoris. SIGNIFICANCE AND IMPACT OF THE STUDY:This work provides an efficient method for identification of lactococcal strains of industrial importance. |
| 巻・号 | 52(5) |
| ページ | 491-6 |
| 公開日 | 2011-5-1 |
| DOI | 10.1111/j.1472-765X.2011.03028.x |
| PMID | 21299580 |
| MeSH | Bacteriological Techniques / methods* DNA Primers / genetics* Lactococcus / classification Lactococcus / genetics* Lactococcus lactis / classification Lactococcus lactis / genetics Polymerase Chain Reaction* RNA, Ribosomal, 16S / genetics* Reproducibility of Results Sensitivity and Specificity Species Specificity |
| IF | 2.173 |
| 引用数 | 10 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1120 JCM 1131 JCM 1136 JCM 1180 JCM 1248 JCM 1290 JCM 1296 JCM 1471 JCM 1649 JCM 2012 JCM 2179 JCM 5176 JCM 5705 JCM 5706 JCM 5803 JCM 5804 JCM 5824 JCM 5826 JCM 5827 JCM 5828 JCM 7638 JCM 8130 JCM 9498 JCM 10343 JCM 11017 JCM 11019 JCM 11040 JCM 11056 JCM 13265 JCM 13471 JCM 13472 JCM 16395 JCM 16647 JCM 17100 JCM 20101 JCM 20128 JCM 20312 JCM 20399 |