Reference - Detail
| RRC ID | 17450 |
|---|---|
| Author | Sawa N, Okamura K, Zendo T, Himeno K, Nakayama J, Sonomoto K. |
| Title | Identification and characterization of novel multiple bacteriocins produced by Leuconostoc pseudomesenteroides QU 15. |
| Journal | J Appl Microbiol |
| Abstract |
AIM:To characterize novel multiple bacteriocins produced by Leuconostoc pseudomesenteroides QU 15. METHODS AND RESULTS:Leuconostoc pseudomesenteroides QU 15 isolated from Nukadoko (rice bran bed) produced novel bacteriocins. By using three purification steps, four antimicrobial peptides termed leucocin A (ΔC7), leucocin A-QU 15, leucocin Q and leucocin N were purified from the culture supernatant. The amino acid sequences of leucocin A (ΔC7) and leucocin A-QU 15 were identical to that of leucocin A-UAL 187 belonging to class IIa bacteriocins, but leucocin A (ΔC7) was deficient in seven C-terminal residues. Leucocin Q and leucocin N are novel class IId bacteriocins. Moreover, the DNA sequences encoding three bacteriocins, leucocin A-QU 15, leucocin Q and leucocin N were obtained. CONCLUSIONS:These bacteriocins including two novel bacteriocins were identified from Leuc. pseudomesenteroides QU 15. They showed similar antimicrobial spectra, but their intensities differed. The C-terminal region of leucocin A-QU 15 was important for its antimicrobial activity. Leucocins Q and N were encoded by adjacent open reading frames (ORFs) in the same operon, but leucocin A-QU 15 was not. SIGNIFICANCE AND IMPACT OF STUDY:These leucocins were produced concomitantly by the same strain. Although the two novel bacteriocins were encoded by adjacent ORFs, a characteristic of class IIb bacteriocins, they did not show synergistic activity. |
| Volume | 109(1) |
| Pages | 282-91 |
| Published | 2010-7-1 |
| DOI | 10.1111/j.1365-2672.2009.04653.x |
| PII | JAM4653 |
| PMID | 20070442 |
| MeSH | Amino Acid Sequence Bacteriocins / chemistry* Bacteriocins / isolation & purification* Base Sequence DNA, Bacterial / genetics Leuconostoc / genetics Leuconostoc / metabolism* Molecular Sequence Data Sequence Analysis, DNA Sequence Analysis, Protein |
| IF | 3.066 |
| Times Cited | 22 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Resource | |
| General Microbes | JCM 1157 JCM 1465 JCM 2257 JCM 2504 JCM 5803 JCM 5885 JCM 6124 |