Reference - Detail
| RRC ID | 17452 |
|---|---|
| Author | Arakawa K, Kawai Y, Ito Y, Nakamura K, Chujo T, Nishimura J, Kitazawa H, Saito T. |
| Title | HPLC purification and re-evaluation of chemical identity of two circular bacteriocins, gassericin A and reutericin 6. |
| Journal | Lett Appl Microbiol |
| Abstract |
AIM:The study aimed for the complete purification and recharacterization of the highly hydrophobic circular bacteriocins, gassericin A and reutericin 6. METHODS AND RESULTS:Gassericin A and reutericin 6 were purified to homogeneity using previously described method and reverse-phase HPLC with an octyl column and eluents of aqueous acetonitrile and 2-propanol. Mass analysis, N-terminal sequencing and bacteriocin assay of the HPLC-purified bacteriocins showed the two bacteriocins had identical seamless circular structures with the same m/z value (5651) of [M + H](+) and both had the same specific activity. D/L-amino acid composition analysis using two distinct methods with the chiral fluorescent derivatization reagents (+)-1-(9-fluorenyl)ethyl chloroformate and O-phthalaldehyde/N-acetyl-L-cystein revealed neither gassericin A nor reutericin 6 contained D-alanine residues contrary to our previous results. CONCLUSION:Purified gassericin A and reutericin 6 are chemically identical circular molecules containing no D-alanine residues. SIGNIFICANCE AND IMPACT OF THE STUDY:The HPLC conditions developed in this study will facilitate advanced purification and correct characterization of other highly hydrophobic bacteriocins. |
| Volume | 50(4) |
| Pages | 406-11 |
| Published | 2010-4-1 |
| DOI | 10.1111/j.1472-765X.2010.02810.x |
| PII | LAM2810 |
| PMID | 20156306 |
| MeSH | Bacteriocins / chemistry* Bacteriocins / isolation & purification* Chromatography, High Pressure Liquid* Lactobacillus / chemistry Mass Spectrometry |
| IF | 2.173 |
| Times Cited | 21 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Resource | |
| General Microbes | JCM 1002 JCM 1131 JCM 11657 |