| RRC ID |
17463
|
| 著者 |
Yin YJ, Wang BJ, Jiang CY, Luo YM, Jin JH, Liu SJ.
|
| タイトル |
Identification and quantification of mycothiol in Actinobacteria by a novel enzymatic method.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Mycothiol (MSH) was reported to be the dominant low molecular weight thiol in members of the Actinobacteria. In this study, a simple, fast, and sensitive method for qualitative and quantitative determination of MSH molecules was developed based on maleylpyruvate isomerase (MPI) from Corynebacterium glutamicum. The principle of this method is that the activity of MPI from C. glutamicum was dependent on MSH molecules. It was found that this MPI activity displayed a linear response (R (2) = 0.9928) at MSH amounts ranging from 0.12 to 3.98 pmol in the defined assay system. This observation was applied to calculate the MSH levels, and the newly developed method was compared with thiol-specific fluorescent-labeling high-performance liquid chromatography method. Forty-eight genera of Actinobacteria were screened for MSH and 43 genera were reported for MSH occurrence, and the MSH levels in Actinobacteria were determined to be 0.01 to 9.69 μmol/g of residual dry cell weight.
|
| 巻・号 |
88(6)
|
| ページ |
1393-401
|
| 公開日 |
2010-12-1
|
| DOI |
10.1007/s00253-010-2918-3
|
| PMID |
20922372
|
| MeSH |
Actinobacteria / chemistry*
Chemistry Techniques, Analytical / methods*
Corynebacterium glutamicum / enzymology*
Cysteine / analysis*
Glycopeptides / analysis*
Inositol / analysis*
cis-trans-Isomerases / metabolism*
|
| IF |
3.53
|
| 引用数 |
6
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 12329
JCM 14000
JCM 14001 |
