RRC ID 17464
著者 Nagata K, Inatsu S, Tanaka M, Sato H, Kouya T, Taniguchi M, Fukuda Y.
タイトル The bifidogenic growth stimulator inhibits the growth and respiration of Helicobacter pylori.
ジャーナル Helicobacter
Abstract BACKGROUND:Triple therapy with amoxicillin, clarithromycin, and a proton-pump inhibitor is a common therapeutic strategy for the eradication of Helicobacter pylori (H. pylori). However, frequent appearance of clarithromycin-resistant strains is a therapeutic challenge. While various quinones are known to specifically inhibit the growth of H. pylori, the quinone 1,4-dihydroxy-2-naphthoic acid (DHNA) produced by Propionibacterium has strong stimulating effect on Bifidobacterium. We were interested to see whether DHNA could inhibit the growth of H. pylori in in vitro or in vivo experimental setting.
MATERIALS AND METHODS:The minimum inhibitory concentration (MIC) of DHNA was determined by the agar dilution method. The inhibitory action of DHNA on the respiratory activity was measured by using an oxygen electrode. Germ-free mice infected with H. pylori were given DHNA in free drinking water containing 100 μg/mL for 7 days.
RESULTS:DHNA inhibited H. pylori growth at low MIC values, 1.6-3.2 μg/mL. Likewise, DHNA inhibited clinical isolates of H. pylori, resistant to clarithromycin. However, DHNA did not inhibit other Gram negative or anaerobic bacteria in the normal flora of the human intestine. Both H. pylori cellular respiration and adenosine 5'-triphosphate (ATP) generation were dose-dependently inhibited by DHNA. Similarly, the culture filtrates of propionibacterial strains inhibited the growth of H. pylori, and oral administration of DHNA could eradicate H. pylori in the infected germ-free mice.
CONCLUSIONS:The bifidogenic growth stimulator DHNA specifically inhibited the growth of H. pylori including clarithromycin-resistant strains in vitro and its colonization activity in vivo. The bactericidal activity of DHNA was via inhibition of cellular respiration. These actions of DHNA may have clinical relevance in the eradication of H. pylori.
巻・号 15(5)
ページ 422-9
公開日 2010-10-1
DOI 10.1111/j.1523-5378.2010.00789.x
PMID 21083748
MeSH Animals Anti-Bacterial Agents / administration & dosage* Anti-Bacterial Agents / metabolism* Helicobacter pylori / drug effects* Helicobacter pylori / growth & development* Male Mice Mice, Inbred C57BL Microbial Sensitivity Tests Microbial Viability / drug effects Naphthols / administration & dosage* Naphthols / metabolism* Oxygen / metabolism Propionibacterium / metabolism*
IF 4.0
引用数 10
WOS 分野 GASTROENTEROLOGY & HEPATOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 1134 JCM 1149 JCM 1217 JCM 1290 JCM 1296 JCM 5826 JCM 5827 JCM 6421 JCM 6432 JCM 6433 JCM 8540 JCM 9979 JCM 10188