RRC ID 17557
著者 Kaneko T, Serikawa T.
タイトル Successful long-term preservation of rat sperm by freeze-drying.
ジャーナル PLoS One
Abstract BACKGROUND:Freeze-drying sperm has been developed as a new preservation method where liquid nitrogen is no longer necessary. An advantage of freeze-drying sperm is that it can be stored at 4 °C and transported at room temperature. Although the successful freeze-drying of sperm has been reported in a number of animals, the possibility of long-term preservation using this method has not yet been studied.
METHODOLOGY/PRINCIPAL FINDINGS:Offspring were obtained from oocytes fertilized with rat epididymal sperm freeze-dried using a solution containing 10 mM Tris and 1 mM EDTA adjusted to pH 8.0. Tolerance of testicular sperm to freeze-drying was increased by pre-treatment with diamide. Offspring with normal fertility were obtained from oocytes fertilized with freeze-dried epididymal sperm stored at 4 °C for 5 years.
CONCLUSIONS AND SIGNIFICANCE:Sperm with -SS- cross-linking in the thiol-disulfide of their protamine were highly tolerant to freeze-drying, and the fertility of freeze-dried sperm was maintained for 5 years without deterioration. This is the first report to demonstrate the successful freeze-drying of sperm using a new and simple method for long-term preservation.
巻・号 7(4)
ページ e35043
公開日 2012-1-1
DOI 10.1371/journal.pone.0035043
PII PONE-D-11-26039
PMID 22496889
PMC PMC3322169
MeSH Animals Cryopreservation / methods Diamide / administration & dosage Edetic Acid / administration & dosage Female Fertility Freeze Drying* Male Rats Semen Preservation / methods* Tromethamine / administration & dosage
IF 2.74
引用数 23
WOS 分野 REPRODUCTIVE BIOLOGY
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