論文 - 詳細
RRC ID | 17557 |
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著者 | Kaneko T, Serikawa T. |
タイトル | Successful long-term preservation of rat sperm by freeze-drying. |
ジャーナル | PLoS One |
Abstract |
BACKGROUND:Freeze-drying sperm has been developed as a new preservation method where liquid nitrogen is no longer necessary. An advantage of freeze-drying sperm is that it can be stored at 4 °C and transported at room temperature. Although the successful freeze-drying of sperm has been reported in a number of animals, the possibility of long-term preservation using this method has not yet been studied. METHODOLOGY/PRINCIPAL FINDINGS:Offspring were obtained from oocytes fertilized with rat epididymal sperm freeze-dried using a solution containing 10 mM Tris and 1 mM EDTA adjusted to pH 8.0. Tolerance of testicular sperm to freeze-drying was increased by pre-treatment with diamide. Offspring with normal fertility were obtained from oocytes fertilized with freeze-dried epididymal sperm stored at 4 °C for 5 years. CONCLUSIONS AND SIGNIFICANCE:Sperm with -SS- cross-linking in the thiol-disulfide of their protamine were highly tolerant to freeze-drying, and the fertility of freeze-dried sperm was maintained for 5 years without deterioration. This is the first report to demonstrate the successful freeze-drying of sperm using a new and simple method for long-term preservation. |
巻・号 | 7(4) |
ページ | e35043 |
公開日 | 2012-1-1 |
DOI | 10.1371/journal.pone.0035043 |
PII | PONE-D-11-26039 |
PMID | 22496889 |
PMC | PMC3322169 |
MeSH | Animals Cryopreservation / methods Diamide / administration & dosage Edetic Acid / administration & dosage Female Fertility Freeze Drying* Male Rats Semen Preservation / methods* Tromethamine / administration & dosage |
IF | 2.74 |
引用数 | 23 |
WOS 分野 | REPRODUCTIVE BIOLOGY |
リソース情報 | |
ラット |