| RRC ID |
17606
|
| 著者 |
Sawajiri M, Nomura Y, Banik S, Nishikiori R, Sonoda Y, Tanimoto K.
|
| タイトル |
Irradiation effect on osteoclastogenesis stimulated by breast cancer cells.
|
| ジャーナル |
Health Phys
|
| Abstract |
To examine the effects of carbon ion and gamma ray irradiation on cancer-induced osteoclastogenesis, mouse calvaria MC3T3-E1 cells were cultured with conditioned medium from irradiated and non-irradiated MCF7 human breast cancer cells. The authors examined RANKL and OPG mRNA expression in osteoblastic MC3T3-E1 cells following treatment with conditioned MCF7 medium. Co-cultured MC3T3-E1 and bone marrow cells treated with conditioned medium from irradiated MCF7 cells showed decreased numbers of osteoclasts, assessed using TRAP staining. Conditioned medium from control MCF7 cells elevated the RANKL/OPG mRNA ratio in MC3T3-E1 cells; this effect was suppressed by carbon ion irradiation of the MCF7 cells. These data demonstrate that indirect interactions between breast cancer cells and MC3T3-E1 cells induce osteoclastogenesis in vitro through modulation of RANKL expression and that this process is suppressed by carbon ion irradiation.
|
| 巻・号 |
101(3)
|
| ページ |
259-64
|
| 公開日 |
2011-9-1
|
| DOI |
10.1097/HP.0b013e3182118333
|
| PII |
00004032-201109000-00005
|
| PMID |
21799342
|
| MeSH |
3T3 Cells
Animals
Bone Neoplasms / etiology
Bone Neoplasms / metabolism
Bone Neoplasms / pathology
Bony Callus / radiation effects*
Breast Neoplasms / metabolism
Breast Neoplasms / pathology*
Carbon / therapeutic use*
Female
Gamma Rays / therapeutic use*
Gene Expression Regulation / radiation effects
Heavy Ion Radiotherapy
Humans
Mice
Osteogenesis / radiation effects*
RNA, Messenger / metabolism
RNA, Messenger / radiation effects
Receptor Activator of Nuclear Factor-kappa B / metabolism
Receptor Activator of Nuclear Factor-kappa B / radiation effects
|
| IF |
0.853
|
| 引用数 |
1
|
|
WOS 分野
|
NUCLEAR SCIENCE & TECHNOLOGY
PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
ENVIRONMENTAL SCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
