| RRC ID |
17610
|
| Author |
Aida J, Higuchi S, Hasegawa Y, Nagano-Ito M, Hirabayashi Y, Banba A, Shimizu T, Kikuchi A, Saga M, Ichikawa S.
|
| Title |
Up-regulation of ceramide glucosyltransferase during the differentiation of U937 cells.
|
| Journal |
J Biochem
|
| Abstract |
The phorbol ester tetradecanoylphorbol acetate (TPA) induces promyelocytic leukaemia cells to differentiate to macrophage-like cells in vitro. During the course of this differentiation, the cells adhere to the bottom of the culture dish, a process that requires an increase in cell surface glycosphingolipids (GSLs). We examined the cellular content of glucosylceramide (GlcCer), the simplest of the GSLs, in a TPA-treated leukaemia cell line, U937. Following TPA treatment, we observed a 3.5-fold increase in GlcCer levels that was caused by enhanced activity of ceramide glucosyltransferase (GlcT-1), which catalyses ceramide glycosylation. Furthermore, in TPA-treated cell GlcT-1 amounts were increased at both the mRNA and protein levels. We also found decreased activity of lactosylceramide synthase in TPA-treated cells, which could also contribute to the increase in cellular GlcCer content.
|
| Volume |
150(3)
|
| Pages |
303-10
|
| Published |
2011-9-1
|
| DOI |
10.1093/jb/mvr058
|
| PII |
mvr058
|
| PMID |
21558327
|
| MeSH |
Cell Adhesion
Cell Differentiation / drug effects
Cell Line, Tumor
Galactosyltransferases / metabolism
Glucosylceramides / metabolism*
Glucosyltransferases / metabolism*
Humans
Leukemia / enzymology
Leukemia / pathology
Macrophages / pathology
RNA, Messenger / metabolism
Tetradecanoylphorbol Acetate / pharmacology
U937 Cells
|
| IF |
2.476
|
| Times Cited |
3
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
U-937 DE-4(RCB0435) |
