RRC ID |
17619
|
著者 |
Kobayashi N, Saito T, Uematsu T, Kishi K, Toba M, Kohda N, Suzuki T.
|
タイトル |
Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice.
|
ジャーナル |
Int Immunopharmacol
|
Abstract |
Host-defense mechanisms against influenza virus (IFV) infection involve both innate and acquired immunities. Among other components, secretory immunoglobulin A (SIgA) in the airway mucosa plays a particularly pivotal role in preventing IFV infection. Among 150 strains of lactic acid bacteria, Lactobacillus pentosus strain b240 (b240) has the highest IgA-inducing potency in mouse Peyer's patch cells. We previously reported a practical new finding that oral ingestion of nonviable heat-killed b240 elevates salivary IgA secretion in humans. The present study aimed to determine if nonviable b240 can prevent IFV infection in mice. In a BALB/c mouse model infected with lethal levels of IFV A/PR8/34 (H1N1), oral administration of b240 for 3 weeks by gavage prior to IFV infection significantly prolonged the survival period. For IFV infection at nonlethal levels, the infectious titers of IFV in the lungs 7 days after infection were significantly reduced after similar b240 administration. Both anti-IFV IgA and immunoglobulin G titers in bronchoalveolar lavage fluid and plasma on day 7 were significantly higher in the b240-treated group than the control group. The augmentation of the anti-IFV immune response by b240 application was preliminarily confirmed by the elevated production of IFV-driven T-cell factors during mixed lymphocyte reactions with b240-primed splenocytes. These results suggest that oral nonviable heat-killed b240 intake can facilitate protection against IFV infection.
|
巻・号 |
11(2)
|
ページ |
199-203
|
公開日 |
2011-2-1
|
DOI |
10.1016/j.intimp.2010.11.019
|
PII |
S1567-5769(10)00383-8
|
PMID |
21095259
|
MeSH |
Administration, Oral
Animals
Cell Line
Dogs
Epithelial Cells / virology
Female
Hot Temperature*
Immunity, Cellular / immunology
Immunity, Mucosal / immunology
Immunoglobulin A / blood
Immunoglobulin G / blood
Influenza A Virus, H1N1 Subtype* / pathogenicity
Lactobacillus / immunology*
Lung / immunology
Lung / virology
Lymphocytes / immunology
Mice
Mice, Inbred BALB C
Orthomyxoviridae Infections / immunology
Orthomyxoviridae Infections / prevention & control*
|
IF |
3.943
|
引用数 |
54
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
IMMUNOLOGY
|
リソース情報 |
ヒト・動物細胞 |
CTLL-2(RCB0637) |