RRC ID 17619
著者 Kobayashi N, Saito T, Uematsu T, Kishi K, Toba M, Kohda N, Suzuki T.
タイトル Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice.
ジャーナル Int Immunopharmacol
Abstract Host-defense mechanisms against influenza virus (IFV) infection involve both innate and acquired immunities. Among other components, secretory immunoglobulin A (SIgA) in the airway mucosa plays a particularly pivotal role in preventing IFV infection. Among 150 strains of lactic acid bacteria, Lactobacillus pentosus strain b240 (b240) has the highest IgA-inducing potency in mouse Peyer's patch cells. We previously reported a practical new finding that oral ingestion of nonviable heat-killed b240 elevates salivary IgA secretion in humans. The present study aimed to determine if nonviable b240 can prevent IFV infection in mice. In a BALB/c mouse model infected with lethal levels of IFV A/PR8/34 (H1N1), oral administration of b240 for 3 weeks by gavage prior to IFV infection significantly prolonged the survival period. For IFV infection at nonlethal levels, the infectious titers of IFV in the lungs 7 days after infection were significantly reduced after similar b240 administration. Both anti-IFV IgA and immunoglobulin G titers in bronchoalveolar lavage fluid and plasma on day 7 were significantly higher in the b240-treated group than the control group. The augmentation of the anti-IFV immune response by b240 application was preliminarily confirmed by the elevated production of IFV-driven T-cell factors during mixed lymphocyte reactions with b240-primed splenocytes. These results suggest that oral nonviable heat-killed b240 intake can facilitate protection against IFV infection.
巻・号 11(2)
ページ 199-203
公開日 2011-2-1
DOI 10.1016/j.intimp.2010.11.019
PII S1567-5769(10)00383-8
PMID 21095259
MeSH Administration, Oral Animals Cell Line Dogs Epithelial Cells / virology Female Hot Temperature* Immunity, Cellular / immunology Immunity, Mucosal / immunology Immunoglobulin A / blood Immunoglobulin G / blood Influenza A Virus, H1N1 Subtype* / pathogenicity Lactobacillus / immunology* Lung / immunology Lung / virology Lymphocytes / immunology Mice Mice, Inbred BALB C Orthomyxoviridae Infections / immunology Orthomyxoviridae Infections / prevention & control*
IF 3.943
引用数 54
WOS 分野 PHARMACOLOGY & PHARMACY IMMUNOLOGY
リソース情報
ヒト・動物細胞 CTLL-2(RCB0637)