| RRC ID |
17626
|
| 著者 |
Kim BY, Yoon HY, Yun SI, Woo ER, Song NK, Kim HG, Jeong SY, Chung YS.
|
| タイトル |
In vitro and in vivo inhibition of glucocorticoid-induced osteoporosis by the hexane extract of Poncirus trifoliata.
|
| ジャーナル |
Phytother Res
|
| Abstract |
This study was performed to discover a novel herbal therapeutic for effective glucocorticoid-induced osteoporosis (GIO) treatment and further to clarify its molecular mechanism of action. Ethanol or methanol extracts of 68 edible Korean native plants were screened to find effective natural plant sources for the treatment of GIO, and Poncirus trifoliata (L.) (Rutaceae, PT) was selected as a final candidate because of its high inhibitory activity plus its novelty. The hexane extract of PT (PT-H) inhibited apoptotic cell death in dexamethasone-induced osteoblastic cell lines, C3H10T1/2 and MC3T3-E1. In vivo mouse results indicated that PT-H not only had an inhibitory effect on the bone loss caused by glucocorticoid, but also promoted bone formation. The molecular mechanisms behind the effect of PT-H on GIO were further clarified by screening of differentially expressed genes (DEGs) between dexamethasone (Dex)-induced osteoblastic cells with or without PT-H treatment. Finally, it was found that the expression level of AnxA6 in Dex-induced osteoblastic cells and prednisolone (PD)-treated GIO-model mice was significantly decreased by PT-H treatment. These findings suggest that PT-H has a strong in vitro and in vivo inhibitory effect on GIO, and decreased expression of AnxA6 may play a key role in this inhibition.
|
| 巻・号 |
25(7)
|
| ページ |
1000-10
|
| 公開日 |
2011-7-1
|
| DOI |
10.1002/ptr.3373
|
| PMID |
21225901
|
| MeSH |
Animals
Annexin A6 / metabolism
Apoptosis / drug effects
Cell Line
Dexamethasone / adverse effects
Glucocorticoids / adverse effects*
Male
Mice
Mice, Inbred ICR
Osteoblasts / drug effects*
Osteogenesis / drug effects
Osteoporosis / chemically induced
Osteoporosis / drug therapy*
Plant Extracts / pharmacology*
Poncirus / chemistry*
|
| IF |
4.087
|
| 引用数 |
10
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
CHEMISTRY, MEDICINAL
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
