| RRC ID |
17633
|
| Author |
Yamada P, Nemoto M, Shigemori H, Yokota S, Isoda H.
|
| Title |
Isolation of 5-(hydroxymethyl)furfural from Lycium chinense and its inhibitory effect on the chemical mediator release by basophilic cells.
|
| Journal |
Planta Med
|
| Abstract |
The effect of hot water extracts of LYCIUM CHINENSE fruits (LCF) on the β-hexosaminidase (β-hexo) release by IgE sensitized BSA stimulated rat basophilic leukemia (RBL-2H3) cells was investigated. The ethylacetate (EtOAc) layer of the extract has shown an inhibitory effect on β-hexo release from RBL-2H3 cells at the antigen antibody binding stage. The water (H₂O) fraction (EFW) of the chloroform (CHCl₃) extract from the EtOAc layer also inhibited β-hexo release at the same stage in a dose-dependent manner. With column chromatography preparation, proton and carbon nuclear magnetic resonance (¹H and ¹³C NMR) spectra, electron ionization mass spectrometer (EI-MS) spectra, and high-performance liquid chromatography (HPLC) analysis, the active component was determined to be 5-(hydroxymethyl)furfural (5-HMF). Thus, the 5-HMF showed an inhibitory effect on β-hexo release at the antigen-antibody binding stage and the antibody-receptor binding stage. Furthermore, 5-HMF suppressed [Ca²+] I influx in the IgE-sensitized BSA-stimulated RBL-2H3 cells. Our results show that 5-HMF may be useful for the treatment or prevention of type I allergic diseases.
|
| Volume |
77(5)
|
| Pages |
434-40
|
| Published |
2011-3-1
|
| DOI |
10.1055/s-0030-1250402
|
| PMID |
21031336
|
| MeSH |
Animals
Anti-Allergic Agents / pharmacology*
Antigen-Antibody Reactions
Basophils / drug effects
Basophils / enzymology
Basophils / metabolism*
Cell Line, Tumor
Dose-Response Relationship, Drug
Fruit / chemistry
Furaldehyde / analogs & derivatives*
Furaldehyde / isolation & purification
Furaldehyde / pharmacology
Immunoglobulin E / immunology
Leukemia, Basophilic, Acute / metabolism
Lycium / chemistry*
Nuclear Magnetic Resonance, Biomolecular
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Rats
beta-N-Acetylhexosaminidases / metabolism*
|
| IF |
2.687
|
| Times Cited |
24
|
|
WOS Category
|
INTEGRATIVE & COMPLEMENTARY MEDICINE
CHEMISTRY, MEDICINAL
PLANT SCIENCES
PHARMACOLOGY & PHARMACY
|
| Resource |
| Human and Animal Cells |
KU812(RCB0495) |
