| RRC ID |
17643
|
| 著者 |
Oike H, Kobori M, Suzuki T, Ishida N.
|
| タイトル |
Caffeine lengthens circadian rhythms in mice.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Although caffeine alters sleep in many animals, whether or not it affects mammalian circadian clocks remains unknown. Here, we found that incubating cultured mammalian cell lines, human osteosarcoma U2OS cells and mouse fibroblast NIH3T3 cells, with caffeine lengthened the period of circadian rhythms. Adding caffeine to ex vivo cultures also lengthened the circadian period in mouse liver explants from Per2::Luciferase reporter gene knockin mice, and caused a phase delay in brain slices containing the suprachiasmatic nucleus (SCN), where the central circadian clock in mammals is located. Furthermore, chronic caffeine consumption ad libitum for a week delayed the phase of the mouse liver clock in vivo under 12 h light-dark conditions and lengthened the period of circadian locomotor rhythms in mice under constant darkness. Our results showed that caffeine alters circadian clocks in mammalian cells in vitro and in the mouse ex vivo and in vivo.
|
| 巻・号 |
410(3)
|
| ページ |
654-8
|
| 公開日 |
2011-7-8
|
| DOI |
10.1016/j.bbrc.2011.06.049
|
| PII |
S0006-291X(11)01007-2
|
| PMID |
21684260
|
| MeSH |
Animals
Behavior, Animal / drug effects
Caffeine / pharmacology*
Cell Line, Tumor
Central Nervous System Stimulants / pharmacology*
Circadian Rhythm / drug effects*
Humans
Liver / drug effects*
Liver / physiology
Male
Mice
Mice, Inbred BALB C
NIH 3T3 Cells
|
| IF |
2.985
|
| 引用数 |
37
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NIH3T3-3-4(RCB1862) |
