RRC ID 17655
著者 Kamio N, Akifusa S, Yamashita Y.
タイトル Diacylglycerol kinase alpha regulates globular adiponectin-induced reactive oxygen species.
ジャーナル Free Radic Res
Abstract It has previously been reported that the globular form of adiponectin (gAd), mature adipocyte-derived cytokine, induced generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated whether diacylglycerol kinases (DGKs), enzymes functioning in sub-cellular signalling pathways, had a role on gAd-induced ROS generation in RAW 264 cells. Administration of R59022, a specific inhibitor for DGK, reduced gAd-induced ROS generation and NO release. RAW 264 cell expressed DGKα mRNA. Depression of DGKα mRNA by RNA interference significantly reduced the ROS generation in response to gAd treatment. Interestingly, transfection with the DGKα-specific small interfering RNA attenuated the expression level of Nox1 mRNA in gAd-treated RAW 264 cells. In addition, the DGKα knockdown with siRNA suppressed gAd-induced NO release.
巻・号 45(3)
ページ 336-41
公開日 2011-3-1
DOI 10.3109/10715762.2010.532495
PMID 21034360
MeSH Adiponectin / metabolism* Animals Cell Line, Tumor Diacylglycerol Kinase / genetics* Diacylglycerol Kinase / metabolism* Gene Expression Gene Knockdown Techniques Humans Macrophages / metabolism* Mice NADPH Oxidase 1 NADPH Oxidases / genetics Nitric Oxide / metabolism Pyrimidinones / pharmacology RNA Interference RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / pharmacology Reactive Oxygen Species / metabolism* Signal Transduction Thiazoles / pharmacology
IF 2.839
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)