RRC ID 17659
Author Honami T, Shimo T, Okui T, Kurio N, Hassan NM, Iwamoto M, Sasaki A.
Title Sonic hedgehog signaling promotes growth of oral squamous cell carcinoma cells associated with bone destruction.
Journal Oral Oncol.
Abstract Sonic hedgehog (Shh) and its signaling have been identified in several human cancers, and increased levels of its expression appear to correlate with disease progression and metastasis. However, the role of Shh in bone destruction associated with oral squamous cell carcinomas, which frequently invade the maxilla or the mandible, is still unclear. In this study we show that the use of siRNA for Shh to block SHH secreted by SAS oral squamous cell carcinoma cells suppressed the tumor growth and tumor angiogenesis of subcutaneous SAS xenografts in vivo. Moreover, blockade of Shh in SAS cells decreased tumor growth and osteoclast number in a tibial metaphysis mouse model. Significantly, we clearly show that SHH stimulated osteoclast formation in a co-culture system consisting of murine bone stromal ST2 cells and murine CD11b(+) bone marrow cells. These findings suggest that Shh signaling is a potential target for the treatment of oral squamous cell carcinoma associated with bone destruction.
Volume 48(1)
Pages 49-55
Published 2012-1
DOI 10.1016/j.oraloncology.2011.08.026
PII S1368-8375(11)00815-3
PMID 21945071
MeSH Animals Bone Marrow Cells / cytology Bone Marrow Cells / metabolism Bone Neoplasms / metabolism* Bone Neoplasms / pathology Bone Resorption / metabolism* Bone Resorption / pathology CD11b Antigen Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Female Hedgehog Proteins / metabolism* Humans Mice Mice, Inbred BALB C Mice, Nude Mouth Neoplasms / metabolism* Mouth Neoplasms / pathology Neoplasms, Experimental RNA, Small Interfering Reverse Transcriptase Polymerase Chain Reaction Stromal Cells / cytology Stromal Cells / metabolism Tibia / pathology*
IF 3.73
Times Cited 9
Human and Animal Cells ST2 (RCB0224)