論文 - 詳細
| RRC ID | 17660 |
|---|---|
| 著者 | Hsum YW, Yew WT, Hong PL, Soo KK, Hoon LS, Chieng YC, Mooi LY. |
| タイトル | Cancer chemopreventive activity of maslinic acid: suppression of COX-2 expression and inhibition of NF-κB and AP-1 activation in Raji cells. |
| ジャーナル | Planta Med |
| Abstract |
Chronic inflammation is one of the predisposing factors for neoplastic transformation. Targeting inflammation through suppression of the pro-inflammatory pathway by dietary phytochemicals provides an important strategy for cancer prevention. Maslinic acid is a novel natural triterpenoid known to inhibit proliferation and induce apoptosis in some tumor cell lines. Although maslinic acid has cytotoxic and pro-apoptotic effects on cancer cells, the underlying mechanisms of its effects on the inflammatory pathway have yet to be elucidated. It has been reported that abnormal expression of pro-inflammatory enzyme cyclooxygenase-2 (COX-2) causes promotion of cellular proliferation, suppression of apoptosis, enhancement of angiogenesis and invasiveness. In the present study, the suppressive effect of maslinic acid on COX-2 expression and the binding activity of upstream transcription factors NF- κB and AP-1, which are known to regulate COX-2 transcriptional activation, were assessed using Raji cells. The anti-inflammatory action of maslinic acid was benchmarked against oleanolic acid and other standard drugs. Western blot analysis and electrophoretic mobility shift assay (EMSA) were employed to analyze COX-2 expression as well as NF- κB and AP-1 binding activity. Our results showed that maslinic acid suppresses COX-2 expression in a concentration-dependent manner. Likewise, the constitutive nuclear NF- κB (p65) activity as well as phorbol 12-myristate 13-acetate (PMA)- and sodium N-butyrate (SnB)-induced AP-1 binding activity in Raji cells were significantly reduced following treatment with maslinic acid. Since maslinic acid suppresses COX-2 expression in Raji cells at concentrations that also lowered the NF- κB (p65) and AP-1 binding activity, it is possible that the suppression of COX-2 by this natural triterpenoid might be achieved, at least in part, via the NF- κB and AP-1 signaling pathways. |
| 巻・号 | 77(2) |
| ページ | 152-7 |
| 公開日 | 2011-1-1 |
| DOI | 10.1055/s-0030-1250203 |
| PMID | 20669087 |
| MeSH | Anti-Inflammatory Agents / pharmacology Anticarcinogenic Agents / pharmacology* Cell Line, Tumor Cell Proliferation / drug effects Cyclooxygenase 2 / drug effects* Cyclooxygenase 2 / metabolism Electrophoretic Mobility Shift Assay Gene Expression / drug effects Humans Lymphoma / metabolism Lymphoma / pathology Lymphoma / prevention & control NF-kappa B / drug effects* NF-kappa B / metabolism Oleanolic Acid / pharmacology Signal Transduction Transcription Factor AP-1 / drug effects* Transcription Factor AP-1 / metabolism Triterpenes / chemistry Triterpenes / pharmacology* |
| IF | 2.687 |
| 引用数 | 34 |
| WOS 分野 | INTEGRATIVE & COMPLEMENTARY MEDICINE CHEMISTRY, MEDICINAL PHARMACOLOGY & PHARMACY PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAJI(RCB1647) |