| RRC ID |
17662
|
| Author |
Tahara K, Kato Y, Yamamoto H, Kreuter J, Kawashima Y.
|
| Title |
Intracellular drug delivery using polysorbate 80-modified poly(D,L-lactide-co-glycolide) nanospheres to glioblastoma cells.
|
| Journal |
J Microencapsul
|
| Abstract |
We investigated doxorubicin (DOX)-loaded nanospheres (NS) formulated using a biodegradable polymer, poly(D,L-lactide-co-glycolide) (PLGA), for targeted chemotherapy of brain tumours. A nonionic surfactant, polysorbate 80 (P80), was used to modify the surfaces of PLGA NS to improve cellular drug delivery. DOX-loaded PLGA NS were formulated by emulsion solvent diffusion and characterised for DOX encapsulation and in vitro release. The effectiveness of DOX-loaded P80-PLGA NS was investigated in A172 human glioblastoma cells. The drug release pattern was dependent on the pH of the medium. Quantitatively, the cellular uptake of NS was significantly increased by P80 surface modification compared with unmodified NS. Confocal laser scanning microscopy studies revealed that DOX was released from NS following accumulation in the cell nuclei. DOX-loaded P80-PLGA NS could significantly inhibit both DOX efflux from the cells and cell proliferation compared with a DOX solution.
|
| Volume |
28(1)
|
| Pages |
29-36
|
| Published |
2011-1-1
|
| DOI |
10.3109/02652048.2010.522258
|
| PMID |
21171814
|
| MeSH |
Antibiotics, Antineoplastic / administration & dosage*
Antibiotics, Antineoplastic / pharmacokinetics
Antibiotics, Antineoplastic / pharmacology
Brain Neoplasms / drug therapy*
Cell Line, Tumor
Cell Membrane Permeability
Cell Proliferation / drug effects
Doxorubicin / administration & dosage*
Doxorubicin / pharmacokinetics
Doxorubicin / pharmacology
Drug Carriers / chemistry
Glioblastoma / drug therapy*
Humans
Lactic Acid / chemistry*
Nanospheres / chemistry*
Nanospheres / ultrastructure
Polyglycolic Acid / chemistry*
Polylactic Acid-Polyglycolic Acid Copolymer
Polysorbates / chemistry*
|
| IF |
2.287
|
| Times Cited |
21
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
ENGINEERING, CHEMICAL
CHEMISTRY, APPLIED
|
| Resource |
| Human and Animal Cells |
A172(RCB2530) |
