論文 - 詳細
RRC ID | 17665 |
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著者 | Ren G, Wang K, Chang R, Su Y, Wang J, Su J, Han B. |
タイトル | Simultaneous administration of fluoride and selenite regulates proliferation and apoptosis in murine osteoblast-like MC3T3-E1 cells by altering osteoprotegerin. |
ジャーナル | Biol Trace Elem Res |
Abstract |
The receptor activator nuclear factor kappa-B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are important for maintaining the balance between bone formation and resorption. However, the regulation of microelements on these factors remains unclear. In this study, we used murine osteoblast-like MC3T3-E1 cells to examine the impact of sodium fluoride (NaF) and/or sodium selenite (Na2SeO3) on the OPG/RANKL system. MC3T3-E1 cells were treated with OPG or RANKL siRNA (or left untreated), and subsequently divided into a control group and five experimental groups, which were exposed to different concentrations of NaF and/or Na2SeO3, and subsequently analysed at 24 h. In particular, we examined cell viability, OPG and RANKL mRNA and protein expression, caspase-3 activity, and the cell cycle of the various cell groups. In summary, our findings suggest that the administration of NaF and/or Na2SeO3 affects the expression of OPG in osteoblast-like MC3T3-E1 cells, thereby contributing to the proliferation and apoptosis induced by the OPG. |
巻・号 | 144(1-3) |
ページ | 1437-48 |
公開日 | 2011-12-1 |
DOI | 10.1007/s12011-011-9130-x |
PMID | 21739163 |
MeSH | 3T3 Cells Animals Apoptosis / drug effects* Blotting, Western Caspase 3 / metabolism Cell Cycle / drug effects Cell Proliferation / drug effects* Coloring Agents Flow Cytometry Fluorides / pharmacology* Mice Osteoblasts / drug effects* Osteoblasts / metabolism* Osteoprotegerin / genetics Osteoprotegerin / metabolism RANK Ligand / biosynthesis RANK Ligand / genetics RANK Ligand / metabolism RNA Interference RNA, Small Interfering / pharmacology Real-Time Polymerase Chain Reaction Sodium Selenite / pharmacology* Tetrazolium Salts Thiazoles |
IF | 2.639 |
引用数 | 6 |
WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | MC3T3-E1(RCB1126) |