| RRC ID |
17667
|
| Author |
Tahara K, Sakai T, Yamamoto H, Takeuchi H, Hirashima N, Kawashima Y.
|
| Title |
Improvements in transfection efficiency with chitosan modified poly(DL-lactide-co-glycolide) nanospheres prepared by the emulsion solvent diffusion method, for gene delivery.
|
| Journal |
Chem Pharm Bull (Tokyo)
|
| Abstract |
This study sought to evaluate the in vitro transfection efficiency of plasmid DNA (pDNA)-loaded chitosan-modified poly(DL-lactide-co-glycolide) nanospheres (CS-PLGA NS) in a gene-delivery system. Using the emulsion solvent diffusion (ESD) method, pDNA-loaded PLGA NS was prepared and the surface of the PLGA NS was modified by binding to CS. Gene transfection ability of CS-PLGA NS was examined in A549 cells. The luciferase gene was used as a reporter gene. The pattern of luciferase activity by pDNA-loaded CS-PLGA NS was initially weak, but gradually grew stronger before decreasing activity. These phenomena should be in accordance with the sustained-release profile of pDNA from PLGA NS in the cytosol and the pDNA protection against DNase. Positively charged CS-PLGA NS was found, by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, not to exhibit cytotoxicity on A549 cells. These results suggest that CS-PLGA NS are potential contributors to efficient pDNA delivery due to their increased interactions with cells and lack of cytotoxic effects.
|
| Volume |
59(3)
|
| Pages |
298-301
|
| Published |
2011-1-1
|
| DOI |
10.1248/cpb.59.298
|
| PII |
JST.JSTAGE/cpb/59.298
|
| PMID |
21372409
|
| MeSH |
Cell Line, Tumor
Chitosan / chemistry*
Diffusion
Emulsions / chemistry
Humans
Lactic Acid / administration & dosage
Lactic Acid / chemistry*
Lactic Acid / toxicity
Nanospheres / chemistry*
Plasmids / chemistry
Plasmids / metabolism
Polyglycolic Acid / administration & dosage
Polyglycolic Acid / chemistry*
Polyglycolic Acid / toxicity
Polylactic Acid-Polyglycolic Acid Copolymer
Solvents / chemistry
Transfection / methods
|
| IF |
1.416
|
| Times Cited |
13
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
CHEMISTRY, MEDICINAL
CHEMISTRY, MULTIDISCIPLINARY
|
| Resource |
| Human and Animal Cells |
A549(RCB0098) |
