RRC ID 17686
著者 Morimoto R, Yamamoto A, Akimoto Y, Obinata A.
タイトル Homeoprotein Hex is expressed in mouse developing chondrocytes.
ジャーナル J Biochem
Abstract Endochondral ossification is a complex process involving the formation of cartilage and the subsequent replacement by mineralized bone. Although the proliferation and differentiation of chondrocytes are strictly regulated, the molecular mechanisms involved are not completely understood. Here, we show that a divergent-type homeobox gene, hematopoietically expressed homeobox gene (HEX), is expressed in mouse chondrogenic cell line ATDC5. The expression of Hex protein drastically increased during differentiation. The chondrogenic differentiation-enhanced expression of Hex protein was also observed in chondrocytes in the tibia of embryonic day 15.5 (E15.5) mouse embryos. The localization of Hex protein in the chondrocytes of the tibia changed in association with maturation; namely, there was Hex protein in the cytoplasm near the endoplasmic reticulum (ER) in resting chondrocytes, which moved to the nucleus in prehypertrophic chondrocytes, and thereafter entered the ER in hypertrophic chondrocytes. These results suggest Hex expression and subcellular localization are associated with chondrocyte maturation.
巻・号 150(1)
ページ 61-71
公開日 2011-7-1
DOI 10.1093/jb/mvr039
PII mvr039
PMID 21454303
MeSH Animals Cartilage / growth & development Cartilage / metabolism Cell Differentiation / genetics* Cell Line Cell Nucleus / metabolism Chondrocytes / cytology* Chondrocytes / metabolism* Chondrogenesis / genetics* Endoplasmic Reticulum / metabolism Gene Expression Regulation, Developmental* Homeodomain Proteins / genetics* In Situ Hybridization / methods Mice Transcription Factors / genetics*
IF 2.476
引用数 1
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)