Reference - Detail
RRC ID | 17688 |
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Author | Hayama T, Tabata K, Uchiyama T, Fujimoto Y, Suzuki T. |
Title | Ferrearin C induces apoptosis via heme oxygenase-1 (HO-1) induction in neuroblastoma. |
Journal | J Nat Med |
Abstract |
We investigated the cytotoxicity of six neolignans (1-6), which are similar in structure to furanocyclohexenone with an angular allyl group, in human neuroblastoma cell lines (IMR-32, LA-N-1, NB-39, SK-N-SH) and normal cells [human umbilical vein endothelial cells (HUVEC) and human dermal fibroblasts (HDF)] using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Two neolignans, ferrearin C (1) and 2, showed significant cytotoxicity in neuroblastoma cells. Typical morphologic features of apoptosis were observed for the ferrearin-type neolignans using Hoechst 33342, and apoptotic cytoplasmic membrane inversion was also induced by ferrearin-type neolignans in IMR-32. Furthermore, a Proteome Profiler Array showed that the ferrearin-type neolignans induced the marked expression of heme oxygenase-1 (HO-1). In a western blot analysis, ferrearin C (1) increased the level of Bax and reduced the level of survivin, indicating the activation of the mitochondrial pathway of apoptosis. |
Volume | 65(3-4) |
Pages | 431-9 |
Published | 2011-7-1 |
DOI | 10.1007/s11418-011-0514-1 |
PMID | 21327520 |
MeSH | Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Apoptosis / drug effects* Blotting, Western Cell Line Cell Line, Tumor Flow Cytometry Heme Oxygenase-1 / genetics Heme Oxygenase-1 / metabolism* Humans Inhibitor of Apoptosis Proteins / genetics Inhibitor of Apoptosis Proteins / metabolism Lignans / chemistry Lignans / pharmacology* Models, Biological Molecular Structure Neuroblastoma / metabolism* Survivin bcl-2-Associated X Protein / genetics bcl-2-Associated X Protein / metabolism |
IF | 2.055 |
Times Cited | 5 |
WOS Category | PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL |
Resource | |
Human and Animal Cells | LA-N-2(RCB0484) SK-N-SH(RCB0426) |