| RRC ID |
1769
|
| 著者 |
Oka A, Aoto T, Totsuka Y, Takahashi R, Ueda M, Mita A, Sakurai-Yamatani N, Yamamoto H, Kuriki S, Takagi N, Moriwaki K, Shiroishi T.
|
| タイトル |
Disruption of genetic interaction between two autosomal regions and the X chromosome causes reproductive isolation between mouse strains derived from different subspecies.
|
| ジャーナル |
Genetics
|
| Abstract |
Reproductive isolation that initiates speciation is likely caused by incompatibility among multiple loci in organisms belonging to genetically diverging populations. Laboratory C57BL/6J mice, which predominantly originated from Mus musculus domesticus, and a MSM/Ms strain derived from Japanese wild mice (M. m. molossinus, genetically close to M. m. musculus) are reproductively isolated. Their F1 hybrids are fertile, but successive intercrosses result in sterility. A consomic strain, C57BL/6J-ChrX(MSM), which carries the X chromosome of MSM/Ms in the C57BL/6J background, shows male sterility, suggesting a genetic incompatibility of the MSM/Ms X chromosome and other C57BL/6J chromosome(s). In this study, we conducted genomewide linkage analysis and subsequent QTL analysis using the sperm shape anomaly that is the major cause of the sterility of the C57BL/6J-ChrX(MSM) males. These analyses successfully detected significant QTL on chromosomes 1 and 11 that interact with the X chromosome. The introduction of MSM/Ms chromosomes 1 and 11 into the C57BL/6J-ChrX(MSM) background failed to restore the sperm-head shape, but did partially restore fertility. This result suggests that this genetic interaction may play a crucial role in the reproductive isolation between the two strains. A detailed analysis of the male sterility by intracytoplasmic sperm injection and zona-free in vitro fertilization demonstrated that the C57BL/6J-ChrX(MSM) spermatozoa have a defect in penetration through the zona pellucida of eggs.
|
| 巻・号 |
175(1)
|
| ページ |
185-97
|
| 公開日 |
2007-1-1
|
| DOI |
10.1534/genetics.106.062976
|
| PII |
genetics.106.062976
|
| PMID |
17057240
|
| PMC |
PMC1775014
|
| MeSH |
Animals
Crosses, Genetic
Female
Fertilization in Vitro
Genetic Linkage
Genotype
Hybridization, Genetic*
Inbreeding
Male
Mice / classification*
Mice / genetics
Mice, Inbred C57BL
Quantitative Trait Loci
Reproduction*
Testis / physiology*
X Chromosome*
Y Chromosome / genetics
|
| IF |
4.015
|
| 引用数 |
56
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 情報 |
Mouse |
